I'm new to RNA-Seq analysis and think this question must have been asked
before, but I can't find an answer in the Galaxy-user or Seq-answers
archives.
My question is, if I use the public Galaxy server interface to TopHat and
Cufflinks, is there any access to cuffmerge?
Also, I'm trying to
Yao,
It's difficult to tell what's wrong without seeing your analysis. However, you
may want to use the reference annotation during the Cufflinks phase to either
estimate isoform expression or guide assembly (this option will appear on our
public server soon). Read the Cufflinks documentation
Carol,
My question is, if I use the public Galaxy server interface to TopHat and
Cufflinks, is there any access to cuffmerge?
No, Cuffmerge is not available in Galaxy.
Also, I'm trying to understand the difference between using cuffmerge and
then using cuffcompare (without a reference
Hi Jeremy,
I tried to run cufflinks to assemble transcripts after running Tophat against
my own reference. This error was encountered.
What was wrong? How to fix it?
Error running cufflinks. [21:01:14] Inspecting reads and determining fragment
length distribution.
Processed 11130 loci.
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