Re: [galaxy-user] rna-seq mutation detection

2011-08-30 Thread Jeremy Goecks
Rich, You can convert base quality scores using the FASTQ Groomer tool. Note that Galaxy tools typically work with Sanger (Phred+33) quality scores. Good luck, J. On Aug 29, 2011, at 10:48 PM, Richard Mark White wrote: > Hi, > Thanks very much. I've tried this, but one thing I have noticed

Re: [galaxy-user] aaChanges

2011-08-30 Thread Jennifer Jackson
Hello David, The main hub for administration tasks is: http://galaxyproject.org/Admin To set up data, please see "Data Integration" and "NGS Local Setup". If this is just your own local instance, you may not need to follow all of the procedures. And you are correct, any log in would be specifi

[galaxy-user] user specific access/options

2011-08-30 Thread Petr Novak
Hi everybody, I am developing the application on Galaxy server. One of the requirement is to create user specific list of options. Is it possible to access somehow $__user_email__ in tag or in ?. I did not found documentation how to used cheetah in galaxy tool xml files but from files provided

[galaxy-user] install tools on cluster

2011-08-30 Thread Wei,Xintao
Hi, I am learning how to install and configure Galaxy on our computer cluster. Many third party tools, for example, bowtie, samtools, R and so on, have to be installed and configured in order to install galaxy. Do I have to convert the third party tools to MPI version in order to run the tools

[galaxy-user] annotation file for cufflinks

2011-08-30 Thread Peng, Tao
Hi jen and galaxy users, when I try to use cufflinks in GALAXY and select "use reference annotation", I am NOT sure how to get the annotation file in GTF format for cufflinks. I was trying to down-load the annotation file from Illumina donated package in Cufflink website, I am NOT sure this is

[galaxy-user] Flagstat crashes in non-linear workflows with TORQUE

2011-08-30 Thread Andrew Warren
>Sorry this is old but I tried recompiling torque after setting the >NCONNECTS to 20 and the issue's still there. > >But there's more: It doesn't affect only flagstat but other non-linear >workflows. One of the two jobs that are submitted when their "father" >stopped running triggers the same error

Re: [galaxy-user] annotation file for cufflinks

2011-08-30 Thread vasu punj
If using Ensembl make sure it is properly formatted. Vasu --- On Tue, 8/30/11, Peng, Tao wrote: From: Peng, Tao Subject: [galaxy-user] annotation file for cufflinks To: "Jennifer Jackson" , "galaxy-user" Date: Tuesday, August 30, 2011, 1:52 PM Hi jen and galaxy users, when I try to use cu

[galaxy-user] August 30, 2011 Galaxy Development News Brief

2011-08-30 Thread Jennifer Jackson
August 30, 2011 Galaxy Development News Brief http://galaxyproject.org/wiki/News%20Briefs/2011_08_30 Source: http://getgalaxy.org new: % hg clone http://www.bx.psu.edu/hg/galaxy galaxy-dist upgrade: % hg pull -u -r 949e4f5fa03a What's New ISMB/ECCB, BOSC, and 3DSIG 2011 Slides

Re: [galaxy-user] annotation file for cufflinks

2011-08-30 Thread vasu punj
UCSC should work fine.   Vasu --- On Tue, 8/30/11, Peng, Tao wrote: From: Peng, Tao Subject: RE: [galaxy-user] annotation file for cufflinks To: "vasu punj" , "Jennifer Jackson" , "galaxy-user" Date: Tuesday, August 30, 2011, 10:53 PM Hi I have down-loaded UCSC HG19 annotation files from

Re: [galaxy-user] annotation file for cufflinks

2011-08-30 Thread Peng, Tao
In the cufflink site, they have annotation from ucsc,ncbi & ensemble. not sure which one to download and then load into galaxy? thanks, Tao Sent from my iPhone On Aug 30, 2011, at 5:52 PM, "vasu punj" wrote: > If using Ensembl make sure it is properly formatted. > Vasu > > --- On Tue, 8/30/11

Re: [galaxy-user] annotation file for cufflinks

2011-08-30 Thread Peng, Tao
Hi I have down-loaded UCSC HG19 annotation files from Cufflinks; Should I just load genes.GTF file (about 100 MB) to galaxy for cufflink analysis? Thanks, tao -Original Message- From: vasu punj [mailto:pu...@yahoo.com] Sent: Tue 8/30/2011 5:52 PM To: Jennifer Jackson; galaxy-user; Pen

Re: [galaxy-user] why the result files are empty ? and is java applet supported by galaxy system?

2011-08-30 Thread liyanhui607
Hi Jelle, Thank you very much for your help ! Best! Yan-Hui Li At 2011-08-26 16:09:16,"Jelle Scholtalbers" wrote: >Dear Yan-Hui Li, > >you have to make sure the output files (and location) are named by >Galaxy. It isn't enough to just have the output files in the right >output directory.