Re: [galaxy-user] How to do BLAST on Galaxy sever?

2012-02-09 Thread Jennifer Jackson
Hi Peter, It looks to me like there are versions both, with the Tool Shed version having the more recent date stamp. But if those extra functions are not needed, then yes, you are correct, the version included in the distribution would be enough. Please send corrections if I have any of this

Re: [galaxy-user] How to do BLAST on Galaxy sever?

2012-02-09 Thread Peter Cock
On Thursday, February 9, 2012, Jennifer Jackson wrote: > Hi David, > > It sounds like you are using the public Galaxy server at > http://usegalaxy.org (http://main.g2.bx.psu.edu)? A > BLAST service is not available on the public instance. > (Megablast is available, but for NGS query data versus >

Re: [galaxy-user] How to do BLAST on Galaxy sever?

2012-02-09 Thread Jennifer Jackson
Hi David, It sounds like you are using the public Galaxy server at http://usegalaxy.org (http://main.g2.bx.psu.edu)? A BLAST service is not available on the public instance. (Megablast is available, but for NGS query data versus a specific set of target native genomes). Bjoern is correct tha

Re: [galaxy-user] How to get reads counts from cufflins?

2012-02-09 Thread Luciano Cosme
Hi Victor, I also wanted to get the count reads but it did not work with Cufflinks. You can use GenomicRanges package (Bioconductor). I am also using Deseq (negative binomial distribution) to detect the differentially expressed genes from time series data. You can find it here http://b

Re: [galaxy-user] user/groups/roles/permissions ???

2012-02-09 Thread Jennifer Jackson
Hello Bernd, This wiki explains the organization of roles in Galaxy: http://wiki.g2.bx.psu.edu/Learn/Security%20Features And if you need help with the Admin UI, this wiki can help: http://wiki.g2.bx.psu.edu/Admin/Interface Most administration help located in the wiki organized under this sectio

Re: [galaxy-user] How to do BLAST on Galaxy sever?

2012-02-09 Thread Björn Grüning
Hi David, at first you need to predict some genes. In the tool-shed you will find some gene prediction tools. With these gene you can search against local BLAST-databases using the NCBI BLAST+ tools, included in Main-Galaxy. Hope that helps, Bjoern > Hi, Everybody, > > I uploaded two complete g

[galaxy-user] How to do BLAST on Galaxy sever?

2012-02-09 Thread David PANG
Hi, Everybody, I uploaded two complete genome sequences and hope to BLAST homologs from both genome. Does anybody know how to do BLAST search on galaxy? Thanks David ___ The Galaxy User list should be used for the discussion of Galaxy anal