Hello,
I am trying to upload 27.2 GB fastq file in Galaxy through FTP, but it
throws an error:
netout: Connection reset by peer
421 Service not available,remote server has closed connection.
Please help.
Thank you,
Sujata
___
The Galaxy Us
Hi Loraine,
I'm glad this was helpful! Linking in the reference annotation can be
one of the biggest hurdles when beginning an RNA-analysis project.
Extracting GTF data directly from the UCSC table browser is currently
possible (and is what was used in the RNA-seq tutorial link I shared),
bu
Hi Lilach,
It seems you ran into a bug that was introduced in the most recent CloudMan
update. It's been fixed now.
If you still have your instance up, the way to get around the issue is to
simply strip off '/cloud' part of the URL and you'll get to Galaxy. Any new
instances will work fine.
Thanks
Hello Kristen,
Our RNA-seq tutorial and FAQ can help out with the general workflow:
https://main.g2.bx.psu.edu/u/jeremy/p/galaxy-rna-seq-analysis-exercise
https://main.g2.bx.psu.edu/u/jeremy/p/transcriptome-analysis-faq
And an iGenomes reference annotation GTF dataset for mm9 is in the
Shared
and I terminated a created from the beginning new instanced for the last
several hours (at least 5 different instances). All don't work.
Lilach
2012/6/14 Lilach Friedman
> Hi,
> Can somebody please help me?
> I'm trying for hours to connect to Galaxy on Amazon EC2. I did everything
> accordin
Hello Amit,
There is no tool to perform this task as a stand-alone function.
However, using a annotation/mapping tool such as Blastn on a local or
cloud instance and a combination of Galaxy's comparison and filter tools
(such as those in the group " Operate on Genomic Intervals") could
likely
Hello,
Galaxy Main
1.) I am having trouble adding annotations to my Tophat and Cufflinks
tools.
I used the Mus.Musculus 9MM reference using the *built in index. *For the
Tophat mapping but no annotations were available in the output files.
I then tried converting the the Ref Genome from the UCSC
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