[galaxy-user] hg19 with rCRS

2013-07-03 Thread rabab khairat
Dear Galaxy,Before the new modifications, i was using hg19 human genome with the rCRS mitochondrial genome for mapping. but now i can't recognize it from the hg19 genomes in the genomes drop-down list.could you please help me, which one is with rCRS.my kind regards Rabab Khairat

[galaxy-user] How to define the cutoff value of RPKM for expressed genes?

2013-07-03 Thread Hoang, Thanh
Hi all, I have been working on RNA-seq data analysis using TopHat and Cuffdiff. One of the problem I have is to define the cutoff RPKM value to tell whether a gene is expressed from the background noise?. Could anybody give me a suggestion? Thank you Thanh

Re: [galaxy-user] How to define the cutoff value of RPKM for expressed genes?

2013-07-03 Thread Jeremy Goecks
The confidence intervals provided by Cufflinks/Cuffdiff are a good place to start; any confidence interval that includes 0 should be looked on skeptically. Good luck, J. On Jul 3, 2013, at 2:52 PM, Hoang, Thanh wrote: > Hi all, > I have been working on RNA-seq data analysis using TopHat and Cuf