Re: [galaxy-user] cuffmerge

2014-02-12 Thread Jennifer Jackson
Hello Janice, Are you using the public Main Galaxy instance at http://usegalaxy.org? Would you be able to submit this as a bug report? Thanks, Jen Galaxy team On 2/10/14 11:07 AM, Janice Patterson wrote: I am analyzing RNA-seq data and I ran Cufflinks with the genes.gtf as a reference ann

Re: [galaxy-user] Error running the Picard tool?

2014-02-12 Thread Jennifer Jackson
Hi Tania, The data is RNA? Did you filter for properly mapped pairs first ('Filter SAM')? I am guessing not, and that is where the error about single end data is coming from, and possibly other insert size values that are skewing the mean. If you plan on using the Tuxedo pipeline, you might

Re: [galaxy-user] Depth of coverage error

2014-02-12 Thread Jennifer Jackson
Hello Sandrine, The sequence identifiers are a mismatch between the bam file and the custom reference genome. https://wiki.galaxyproject.org/Support#Reference_genomes I'll reply with more detail to your bug report about the same issue. Best, Jen Galaxy team On 2/10/14 6:09 AM, Sandrine Imbe

Re: [galaxy-user] Picrust upload

2014-02-12 Thread Jennifer Jackson
Hello Ines, You have reached the support mailing list for the public Main Galaxy instance at http://usegalaxy.org. While we can help with many usage questions, sometimes for specific tools it is best to contact the tool author and/or lab running the instance hosting the tool. This is one of t

Re: [galaxy-user] Questions regarding Circster visualization

2014-02-12 Thread Friederike Dündar
Dear Jeremy, thanks for the reply! Indeed, there's another feature I don't fully understand: I have a bgiWig file that contains reads of only one chromosome. I expected that Circster would display this one chromosome as one circle, but apparently Circster always draws a circle where all possible

Re: [galaxy-user] Questions regarding Circster visualization

2014-02-12 Thread Jeremy Goecks
> Indeed, there's another feature I don't fully understand: I have a bgiWig > file that contains reads of only one chromosome. I expected that Circster > would display this one chromosome as one circle, but apparently Circster > always draws a circle where all possible chromosomes of a genome ar