Re: [galaxy-user] Question about output CuffDiff SplicingDiff

2011-06-16 Thread Rory Kirchner
What version of Cufflinks is your Galaxy installation running? A recent version (1.00 and 1.01) had a problem that was causing the splicing and promoter use tests to have very few differentially regulated genes, according to http://cufflinks.cbcb.umd.edu/. -rory On Jun 16, 2011, at 8:13 AM, Fe

Re: [galaxy-user] Text Manipulation: Filter out duplicates (uniq) from an plain text file ?

2011-05-06 Thread Rory Kirchner
Is there a reason why just using the command line tool isn't workable for you? Personally, I'm happy when I can just do something quick like that. Also you can simplify your command with sort filename | uniq. -rory On May 6, 2011, at 9:45 AM, Roman Valls wrote: > Hey galaxy users, > > Thats a

Re: [galaxy-user] Gene Name in Cufflink/compare/diff

2011-01-24 Thread Rory Kirchner
For the ensembl annotation, you can download the gtf file from ensembl for your organism here: http://uswest.ensembl.org/info/data/ftp/index.html To use this, you need to fix it because the chromosome names are not correct (depending on your organism, it is not correct for mouse and rat at leas

Re: [galaxy-user] Galaxy for gene expression comparison

2011-01-21 Thread Rory Kirchner
I am not sure about cuffcompare, but cuffdiff doesn't generate any extra files if you add more groups and replicates to the command line. It adds columns to the output files but the number of files remains the same. For a workflow for Martin for now, I would suggest doing this for making calls