Re: [galaxy-user] Any thing wrong with my cufflink process in galaxy?

2011-08-14 Thread Jeremy Goecks
Yao, It's difficult to tell what's wrong without seeing your analysis. However, you may want to use the reference annotation during the Cufflinks phase to either estimate isoform expression or guide assembly (this option will appear on our public server soon). Read the Cufflinks documentation t

[galaxy-user] Any thing wrong with my cufflink process in galaxy?

2011-08-10 Thread yao chen
Dear all: Recently, I run cufflink in galaxy on the internet. I want to compare two samples, However, I found no transcript or gene passed the significant level, even many of them have large FPKM in one sample and 0 FPKM in another sample. Any thoughts? Below is my cufflink process: I have four