Re: [galaxy-user] Cuffdiff tracking file does not report all genes and trancrips from reference annotation?

2012-11-30 Thread Jennifer Jackson
Hello Wei, The results do sound strange. The best advice to start with is to make sure that you are up-to-date with both Galaxy and the RNA-seq tools and using the best possible inputs. 1 . Make sure that you are running the latest distribution

[galaxy-user] Cuffdiff tracking file does not report all genes and trancrips from reference annotation?

2012-11-29 Thread Wei Liao
Hi, Galaxy user. I ran into a problem when using Cuffdiff 1.2.1 in Galaxy local instance to check differential expressed genes in my samples. I have 3 normals and 6 cancers samples, I did the following: - After tophat for each samples, run cufflink with refseq annotation which has 25266 genes and