Re: [galaxy-user] Cufflinks merging more than one transcript on bacterial genomes

2012-01-25 Thread Jeremy Goecks
Noa, > This is one thing I would like help with- is it worth simply reducing to > nothing the max intron size? What is accepted consensus when using tophat on > bacterial genomes? I'm not sure that folks on this list have much experience with bacterial transcriptome analysis. You might try seq

[galaxy-user] Cufflinks merging more than one transcript on bacterial genomes

2012-01-24 Thread Noa Sher
Hi everyone I am running tophat and cufflinks on a bacterial genome.  As parameters for tophat, I used minimal distance between introns as 15bp, and max intron size as 1500bp. Visual verification of this looks decent.  What I mean by this is that when I look at the