Re: [galaxy-user] Extract genomic DNA

2011-12-14 Thread Jeremy Goecks
Rebecca, You should be able to use a custom genome with this tool by selecting "History" from the "Source for Genomic Data" parameter. The bug you're describing has, to the best of my knowledge, been fixed in Galaxy and should not be present anymore. On which Galaxy instance are you seeing thi

[galaxy-user] Extract genomic DNA

2011-12-14 Thread Rebecca C Mueller
Hi all, Does anyone know if you can use the "Extract Genomic DNA" command with a genome not in the database? I am working with an algal genome (C. merolae) that isn't currently in the pulldown Database/Build menu. I keep getting the "Unspecified genome build" error, and am assuming that's the

Re: [galaxy-user] Extract genomic DNA-strand information is not recognized

2011-10-25 Thread Jennifer Jackson
Hi Sarah, One of the specifications of BED format is that the coordinates are with respect to the forward strand. BED format originated at UCSC, and this is their full specification: http://genome.ucsc.edu/FAQ/FAQformat.html#format1 And Galaxy's summary (also on tool forms that accept BED for

[galaxy-user] Extract genomic DNA-strand information is not recognized

2011-10-24 Thread Sarah
Hello, I am trying to extract sequences from a FASTA file containing genomic information. The coordinates are in a tab-delimited format, which is recognized as BED format by Galaxy (meaning that the 6th column is correctly interpreted as 6. Strand). However, upon running "Fetch sequences" , Ex

Re: [galaxy-user] Extract Genomic DNA Problem

2011-06-22 Thread Fabian Owuor
Hi all, i am not sure if this is the right forum. but i need as much access to start up information on the system for developers. we are part of a group in Kenya trying to come up with some apps on a gnome sequencing project we are starting here. regards -- Fabian .J. Owuor > > www.adelphitra

Re: [galaxy-user] Extract Genomic DNA Problem

2011-06-21 Thread Stephen Taylor
Hi jen, In your original dataset, there are extra spaces around the tabs. Where "^I" indicates a tab and "$" indicates an end-of-line character, the entire datafile looks like this: chr5 ^I 47258168 ^I 47259240$ chr18 ^I 1938527 ^I 1939965$ chr2 ^I 101973625 ^I 101974007$ chr4 ^I 75653898 ^I 75

Re: [galaxy-user] Extract Genomic DNA Problem

2011-06-21 Thread Jennifer Jackson
011 10:23 AM To: Stephen Taylor Cc: galaxy-u...@bx.psu.edu Subject: Re: [galaxy-user] Extract Genomic DNA Problem Hi Stephen, In your original dataset, there are extra spaces around the tabs. Where "^I" indicates a tab and "$" indicates an end-of-line character, the entire

Re: [galaxy-user] Extract Genomic DNA Problem

2011-06-21 Thread John David Osborne
From: galaxy-user-boun...@lists.bx.psu.edu [galaxy-user-boun...@lists.bx.psu.edu] On Behalf Of Jennifer Jackson [j...@bx.psu.edu] Sent: Tuesday, June 21, 2011 10:23 AM To: Stephen Taylor Cc: galaxy-u...@bx.psu.edu Subject: Re: [galaxy-user] Extract Genomic DNA Problem Hi Stephen, In your original

Re: [galaxy-user] Extract Genomic DNA Problem

2011-06-21 Thread Jennifer Jackson
Hi Stephen, In your original dataset, there are extra spaces around the tabs. Where "^I" indicates a tab and "$" indicates an end-of-line character, the entire datafile looks like this: chr5 ^I 47258168 ^I 47259240$ chr18 ^I 1938527 ^I 1939965$ chr2 ^I 101973625 ^I 101974007$ chr4 ^I 75653898

Re: [galaxy-user] Extract Genomic DNA Problem

2011-06-21 Thread Stephen Taylor
Hi Jeremy, This is a formatting issue with your input file; it needs to be tab-delimited but it's not currently. You'll need to: (a) convert spaces to tabs using the "Convert delimiters to Tabs" tool; (b) click on the pencil icon and set the data type to BED. Thanks, this works, but as a us

Re: [galaxy-user] Extract Genomic DNA Problem

2011-06-21 Thread Jeremy Goecks
Stephen, This is a formatting issue with your input file; it needs to be tab-delimited but it's not currently. You'll need to: (a) convert spaces to tabs using the "Convert delimiters to Tabs" tool; (b) click on the pencil icon and set the data type to BED. Best, J. On Jun 21, 2011, at 8:45 AM

[galaxy-user] Extract Genomic DNA Problem

2011-06-21 Thread Stephen Taylor
Hi, I was trying to extract FASTA sequences using the following tab separated data for Chicken on the Galaxy Main server: chr5 4725816847259240 chr181938527 1939965 chr2 101973625 101974007 chr4 7565389875674045 chr194258837 4263299 chr4

[galaxy-user] Extract Genomic DNA Problem

2011-06-17 Thread Steve Taylor
Hi, I was trying to extract FASTA sequences using the following tab separated data for Chicken on the Galaxy Main server: chr5 4725816847259240 chr181938527 1939965 chr2 101973625 101974007 chr4 7565389875674045 chr194258837 4263299 chr4

Re: [galaxy-user] extract genomic DNA

2011-02-14 Thread Jeremy Goecks
Hello, We recently updated our main server and a bug was introduced to this tool. This bug has been fixed in our main repository, and we'll update our server in the next day or so to fix this bug. Thanks, J. On Mon, Feb 14, 2011 at 5:24 PM, Keith E. Giles wrote: > a few days ago I was able to

[galaxy-user] extract genomic DNA

2011-02-14 Thread Keith E. Giles
a few days ago I was able to extract genomic DNA from a bed file in either fasta or interval format. But today, that same file, and subsets of that larger file are giving me errors. It doens't tell me much. What is so incredibly frustrating is that the datasets are identical!!! One day they seem