Hello Jianguang,
Your data is paired end, but it was already split into forward and
reverse reads when extracted in FASTQ format from the SRA. The tool
'FASTQ splitter' is not needed (this tool literally cuts a joined
sequence record into two). What you most likely want to do instead is
sort
I have problem to split a paired-end FASTQ dataset into two separate datasets.
In order to explain the problem clearly, I list the detail of what I did with
my dataset:
Step 1) My aim is to compare datasets for the differential alternative
splicing. I downloaded paired-end datasets at FASTQ f
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