Dear All,
I am analysing RNA-seq datasets for the differential splicing events between
cell types. My reads are 36bp long. In order to increase the quality of reads,
I need to trim some nucleotides from ends. How many nucleotides can I trim? I
am afraid that if I trim too much, the reliability
Hello Jianguang,
This general protocol is also in the RNA-seq tutorial:
http://main.g2.bx.psu.edu/u/jeremy/p/galaxy-rna-seq-analysis-exercise
-- Understanding and QCing the reads
That said, I had a sample of your data from before and I ran FastQC on
it and see what you mean, the quality drops
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