[galaxy-user] Please help with the settings for Cufflink, Cuffmerge and Cuffdiff

2012-08-27 Thread Du, Jianguang
Dear All, I am looking for the differential splicing events between cell types. Although I got a lot of helps from Jen and from protocols found online, I am still not sure about some settings for Cufflink, Cuffmerge and Cuffdiff. 1) For Cufflink: There is a setting for Bias Correction. I made

Re: [galaxy-user] Please help with the settings for Cufflink, Cuffmerge and Cuffdiff

2012-08-27 Thread Jennifer Jackson
Jianguang, If your input dataset(s) have a genome assigned (database) and that genome is local to Galaxy (I believe this is true for your data, mm9, correct?), then using bias correction would be an advantageous option to make use of according to our experience and the tool authors in the