I tried agaian and the same problem. I tuned off the bias correction
but
mantained the GFT file. May be this is the problem? I didnt find
your history.
Thanks
Look for the history I've shared in History Options --> Histories
Shared with Me. As requested, if you're still having problems,
, 2011 16:16:14
Asunto: Re: [galaxy-user] Trouble with RNAseq analysis
On Mar 30, 2011, at 3:01 PM, Cristian Rojas wrote:
> Hi Jeremy, I turned off the bias correction and didnt use the genome option,
> just put the refernde (GTF file), but still the problem.
>
> Any help?
>
I
On Mar 30, 2011, at 3:01 PM, Cristian Rojas wrote:
Hi Jeremy, I turned off the bias correction and didnt use the genome
option,
just put the refernde (GTF file), but still the problem.
Any help?
I was able to run Cufflinks successfully with bias correction turned
off; see the history t
:00
Asunto: Re: [galaxy-user] Trouble with RNAseq analysis
Cristian,
The ">" is a formatting character; what needs to match is the string after the
">" in the genome file and the entries in the contig column of your GTF. Your
GTF is quite different that your genome fil
">" names in Genome fasta. What must I do?
> Cristian
>
>
>
> - Mensaje original
> De: Jeremy Goecks
> Para: Cristian Rojas
> CC: galaxy-user@lists.bx.psu.edu
> Enviado: miércoles, 30 de marzo, 2011 12:53:50
> Asunto: Re: [galaxy-user] Troubl
I did it.
> Cristian
>
>
>
> - Mensaje original
> De: Jeremy Goecks
> Para: Cristian Rojas
> CC: galaxy-user@lists.bx.psu.edu
> Enviado: miércoles, 30 de marzo, 2011 12:02:47
> Asunto: Re: [galaxy-user] Trouble with RNAseq analysis
>
> Cristian,
>
Cristian,
Please share your history with me (History Options --> Share/Publish --> Share
with User --> my email) and I'll take a look.
Thanks,
J.
On Mar 30, 2011, at 10:48 AM, Cristian Rojas wrote:
> Hi everybody, I am trying to analyze the differential expression between two
> RNAseq samples
Hi everybody, I am trying to analyze the differential expression between two
RNAseq samples. But I found many troubles aligning my reads. I will describe
what I did. First I groomed the FastQ files (2). Then I uploaded the Sorghum
genome and aligned the reads to it with Tophat. Aftter that, I tr
8 matches
Mail list logo
