Re: [galaxy-user] question on cufflinks output

2011-06-27 Thread Jennifer Jackson
Hello, The error is indicating that the end of your dataset is larger than the chromosome it is aligned to for this line (at least). This is expected when attempting display of certain data types at UCSC. This is a known issue. You can either remove these lines from your dataset with tools i

Re: [galaxy-user] question on cufflinks output

2011-06-27 Thread YBao
Hi galaxy-users When trying to display a set of chip microarray data in UCSC main, , we encounter this error: Error(s): Error line 38879 of http://main.g2.bx.psu.edu/root/display_as?id=3221463&display_app=ucsc&authz_method=display_at: chromEnd larger than chrom chr21_random size (1679928 > 167969

Re: [galaxy-user] question on cufflinks output

2011-06-25 Thread Jeremy Goecks
Hello Wen, It's not necessary to send multiple emails to the mailing list; we track incoming emails to ensure that we respond to all of them. Your FPKM values do look high, but keep in mind that coverage is only part of the FPKM calculation; it's also dependent on transcript length and the tota

[galaxy-user] question on cufflinks output

2011-06-24 Thread Wen Huang
Dear Galaxy team and users, I have a question on the output by cufflinks on Galaxy. I started with about 28M paired-end reads and mapped them to the reference genome using Tophat on Galaxy. The aligned fragments were assembled by cufflinks, again on Galaxy and I got an output with the first few

[galaxy-user] question on cufflinks output

2011-06-24 Thread Wen Huang
Dear Galaxy team and users, I have a question on the output by cufflinks on Galaxy. I started with about 28M paired-end reads and mapped them to the reference genome using Tophat on Galaxy. The aligned fragments were assembled by cufflinks, again on Galaxy and I got an output with the first few