Are you new to the intertubes?
Apparently, you are.
Since that's the case, here's the address to unsubscribe THAT APPEARS AT THE
END OF ALL EMAILS SENT TO HISTONET**.
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Emily
**this includes the three unsubscribe messages you sent to histonet
Dear All:
I am writing to discuss the function of each step in BrdU staining.
As you might know, Citrate buffer retrieval at 95 degree for 30 min, and 2N HCl
for another 30 min at RT are two critical steps for BrdU staining.
(1) For citrate buffer retrieval: Is this really antigen retrieval? Or
please unsubscribe
thank you
___
This email may contain confidential protected health information and/or
proprietary information belonging to the sender that is legally privileged
under local, state, or federal
Actually, i think the conclusion we reached was that the decal was the
problem...
On Fri, Sep 12, 2008 at 12:53 PM, Mark Tarango <[EMAIL PROTECTED]>wrote:
> I had the same problem but our cores were fixed in 10% NBF. I tried
> everything I could think of to get it to work as far as retrieval, s
Hello all,
Is anyone in need of a histotech in the Inland empire area of Southern
California? I will be moving there soon. Thanks!
Jennifer Cresor
(360)274-5292
[EMAIL PROTECTED]
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://li
I had the same problem but our cores were fixed in 10% NBF. I tried
everything I could think of to get it to work as far as retrieval, so I
ended up having to do a real TUNEL assay. We had to assume the antibody
wasn't specific enough. Same antibody and staining platform as you.
Mark
On Fri, S
No it is not the rule. The thing is that sections covered with paraffin will be
isolated from the air and the epitopes will not be readily oxidized. You can
also keep them in a container immersed in mineral oil. If you do either, you do
not need to keep them at low temperature.
René J.
--- On F
Is anyone having problems with IHC staining after a combination os AZF fixation
and decal? There was a recent string on the Histonet regarding B-Plus but I
can't remember exactly what was discussed. We are experiencing problems with
TdT staining. We get nothing on the core biopsies and only edge
I would buy a Sakura autostainer for H&E (it can also run some HC procedures),
but with that work volume you will probably have about 20-25 HC per day and
that amount can be handled cheaply manually.
René J.
--- On Fri, 9/12/08, Rush, Joyce <[EMAIL PROTECTED]> wrote:
From: Rush, Joyce <[EMAIL P
Hi Aprill,
Why do you keep paraffin-embedded slides in refrigerator? Is it the
rule?
Naira
-Original Message-
Message: 1
Date: Fri, 12 Sep 2008 09:50:48 -0700
From: Aprill Watanabe <[EMAIL PROTECTED]>
Subject: [Histonet] Re: Unstained slide storage
To:
Message-ID: <[EMAIL PROTECTED]>
We are a low volume lab and currently do a limited menu of special and
IHC stains on a stainer. We do our H&E's by hand. The stains for our
IHC stainer are getting harder and harder to get. We are considering
sending our IHC's out and getting a stainer for H&E and special stains.
I'd appreciate
Hello:
From my experience the DRS 2000 and the Leica XL are dependable hard
working units. If you have any questions contact me.
Best wishes,
Rick
[EMAIL PROTECTED]
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwes
I will be out of the office starting 09/12/2008 and will not return
until 09/19/2008.
Any histology request contact [EMAIL PROTECTED]
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo
I consistently cut more slides than I need and store them in the
refrigerator after I dip them in melted paraffin. I have seen decreased
antigenicity over years with some antibodies but not all.
Aprill Watanabe, B.S.
Research Associate
Integrated Cancer Genomics Division
Tissue Microarray Center
Under separate cover I am sending a review I wrote on this issue with the
procedure you are looking for.
René J.
--- On Fri, 9/12/08, Cindy DuBois <[EMAIL PROTECTED]> wrote:
From: Cindy DuBois <[EMAIL PROTECTED]>
Subject: [Histonet] KOH for Toenails
To: histonet@lists.utsouthwestern.edu
Date: Fr
Has anyone used (or seen) the KOH method of testing toenails for fungus?
I am looking for a procedure as our pathologists said they want us to start
using this method.
Also, what are the pros and cons of this method?
Cindy
___
Histonet mailing list
His
Thank you everyone for all your excellent ideas for the bottom of a new
stainless steel waterbath!! I can paint it after all which is what I
originally planned to do. I am very grateful for this website since I
work alone and have no one to ask if I have a problem, so I do
appreciate all your res
Could you fix the tissue in osmium tetroxide, similar to what they do in EM?
- Fix first in 10% neutral buffered formalin to preserve morphology of other
cells, rinse in buffer, then fix in 1% OsO4, rinse again in buffer. That
would fix and stain all the fat black.
- Then process the tissue as usu
18 matches
Mail list logo
