Hao,
It might be possible that not all of the mountant has been removed. Though I am
surprised that the staining is not better.
It just might be one of those instances where the keratin epitopes are damaged
during the 5 year storage.
Possibly the phosphotungstic acid, often included in the PAP
Do not bother to retrieve.
Usually PAP smears are not fixed in formalin but are alcohol fixed.
Alcohol is an excellent fixative for intermediate filaments as well as
keratins.
I would just de-coverslip the slides, rinse in buffer, block endogenous
enzyme, add your diluted antibody (you will probabl
Since there is only one mole of ammonium ion in each mole of ammonium chloride,
1 M = 1 N. Thus 0.1 mole of ammonium chloride in 0.1 liter of water is a 1 N
solution. Thus 5.35 g ammonium chloride in 100 ml of water is a 1 N solution.
From: Ramadan El Sokary [EM
Usually PAP smears are NOT fixed with NBF so they should NOT be treated with
HIER before doing IHC. If you have some spare smear do IHC directly (after
destaining).
René J.
--- On Sat, 9/13/08, hao zhang <[EMAIL PROTECTED]> wrote:
From: hao zhang <[EMAIL PROTECTED]>
Subject: [Histonet] antigen
