hi,
1. no idea.
2. yes. if they proliferate.
3. just treat with PBST (Tris-buffered PBS) before your adding of antibody.
4. possibly..just notice some BrdU positive cells could be microglia..be
careful of the leakage..and if you dont have a GFP-rat, have you considered of
using astrocytes from
Thank you everyone, that was very helpful information.
How do you store your unstained slides? Specifically, how do you store them
during the initial period between cutting and sign-out, and, if they are
kept after sign-out, how are they stored, if differently?
We have a not so insignificant
Hi, I am looking for some help with NSE histology staining on frozen
muscle tissue. I would really like a method, with all the little tricks
(if any), that is working well for someone. I have been trying the
method from Dubowitz (Muscle Biopsy, A Practical Approach the Armed
Forces Institute
Hi,
I do not seem to be able to achieve acceptble morphology of perfused rat liver.
I seems as if some of the cytosol of the hepatocytes are washed away as seen
in toluidine blue stained cryo-sections (see image18.jpg). I am to use the
tissue cryo-sections in immunohistochemistry and
We have been cutting our tissue for amyloid staining @ 8 microns. One
of my pathologists heard that 10 microns is now standard and to use a
negative and weakly positive control. Does anyone have any new
information in this area? Thanks ahead of time!
Dorothy Webb, HT (ASCP)
Histology
Dear Tojo:
Your problem could be due to 3 things. 1. Waiting too long to fix
the tissue. 2. Freezing too slowly. 3. Interpreting the lack of staining
of hepatocyte glycogen as an artifact.
1. You are waiting 5 minutes after death to fix the tissue. There is
absolutely NO reason to spend
job in fairhope alabama, beautiful area along the bay. dematologist office
looking for someone to do the mohs surgery. contact anita, providence hosp.
mobile alabama, 251-633-1422.
_
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