Re: [Histonet] Re: Which one first - antigen retrieval or peroxidase block

2008-09-30 Thread Rene J Buesa
As far as I remember enzymes are proteins and are they not supposed to cross-link with formalin also? René J. --- On Mon, 9/29/08, Greg Dobbin [EMAIL PROTECTED] wrote: From: Greg Dobbin [EMAIL PROTECTED] Subject: Re: [Histonet] Re: Which one first - antigen retrieval or peroxidase block To:

[Histonet] RELIA Histology Careers Bulletin 9-30-08

2008-09-30 Thread Pam Barker
Hello Histonetters! It is hard to believe the summer is over already. Before we know it, it will be 2009! Let’s Get The Ball Rolling!!! Looking for a new job in the Fall? Planning a job change after the holidays? Considering making a move in 2009? Are you a histo tech looking for a

[Histonet] Great opportunity for Histotechnician in brand new laboratory!

2008-09-30 Thread Martinez, Angela
Great opportunity for Histotechnician in brand new laboratory! Gastrointestinal Associates of North Texas in Ft Worth , is looking for a certified HT or HTL to run their newly constructed laboratory. Candidate must be ASCP certified and meet CLIA-88 regulations to perform gross dissection. Prior

Re: [Histonet] Lot Logs

2008-09-30 Thread Rene J Buesa
I never did that BUT if you have a problem during any step of your process it is a good practice to consult the supplier with the lot number of their products to find out if the problem you experienced has been reported by others also. René J. --- On Tue, 9/30/08, Matt Roark [EMAIL PROTECTED]

[Histonet] PLS Help with IHC protocol

2008-09-30 Thread Kimberly Tuttle
Step 10: Add antibody at 10ug/ml in PBS + 0.1%BSA + 1:10 volume CAS block Can someone break this down for me in simple instructions? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410)

RE: [Histonet] PLS Help with IHC protocol

2008-09-30 Thread Patsy Ruegg
Kim, We need to know what the concentration of the antibody is before you make it 10ug/ml Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email [EMAIL PROTECTED] web site www.ihctech.net This

RE: [Histonet] Lot Logs

2008-09-30 Thread Patsy Ruegg
No, not sure my supplier even furnishes lot numbers on that. Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email [EMAIL PROTECTED] web site www.ihctech.net This email is confidential and

[Histonet] Control blocks for Special Stains

2008-09-30 Thread Jeanne Clark
We are looking at making our own SS blocks from fresh tissue injected with organisms we can get from our Micro dept.  Any advice on this..what type of fresh tissue?, etc.   Jeanne Jeanne Clark HT/MLT (ASCP) Pathology Manager Mission Hospitals 428 Biltmore Ave. Asheville, NC. 28801

[Histonet] Amyloid Controls

2008-09-30 Thread Beth Delescavage
Hi Everyone~ We are having a hard time finding Amyloid controls. Can anyone recommend a company that has good controls? Thanks~ Beth Beth Delescavage, BS, HTL (ASCP) QIHC DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is

[Histonet] Seeking a Pathology Assistant temp tech as well as a local histologist

2008-09-30 Thread Cheryl
Hi everyone-a couple of temp openings for your viewing enjoyment! Seeking a fully qualified Pathology Assistant (not a histotech who can gross but a PA) for a travel position. Please call for specifics. Also seeking a local temp tech for Western Ohio. We're trying to help a facility that

[Histonet] Colored paraffin

2008-09-30 Thread Cheryl Crowder
Several years ago we purchased colored paraffin from Polysciences. We got both pink and amethyst (purple). They had other colors. The colors were great for small biopsies. However, if we used colored cassettes (green, yellow, lavender, blue) the paraffin darkened and we could hardly see the

[Histonet] Career Opening!

2008-09-30 Thread Evans, Andria B.
We have an opening for a full time Histotechnican/Histotechnologist M-F 8:30am - 5:00pm or 9am-5:30pm, with occasional Saturdays. We have excellent pay, benefits, retirement plan, PTO, and many other perks. We are a non-profit hospital located in York, Pennsylvania. We do about 40,000 cases a

[Histonet] Metanil Yellow and Methyl Green

2008-09-30 Thread Igor Deyneko
Dear Histonetters! I have a question regarding a dye called Metanil Yellow, can anyone suggest a good vendor for this dye (I know Sigma and Polyscientific sell it) and what are your impressions? Second question, I have asked long time regarding Methyl Green. I narrowed it down to DAKO ready to

[Histonet] Histology or IHC conferences/workshops

2008-09-30 Thread Igor Deyneko
Dear Histonetters! I was wondering where someone knows of any useful conferences or workshops , which deal with histological techniques, or Immunohistochemical techniques or even microscopy techniques( I heard about a place in Cape Cod), coming up in 2009??? Any information would be appreciated.