[Histonet] microtome

2008-10-23 Thread Ernestine Middleton
Need suggestion on purchase a microtome that is good for section undecalified bone.  Please email me at : [EMAIL PROTECTED] Thank.   Ernestine Middleton Montefiiore Med. Ct. Bronx, NY l0467 718-920 4157   I using Xpress rapid processor.  I am experience problem with using Warthin starry for HP on

[Histonet] Lipid stain for mammalian photoreceptors

2008-10-23 Thread Rivka Rachel
Is anyone aware of a lipid (or other) stain that would label the outer segments in mammalian photoreceptors in fixed tissue? We are aware of PPD which works well in plastic embedded tissue and are looking for something similar that would work with either paraffin embedded or cryosectioned tissue.

Re:[Histonet] need help for cultured cells

2008-10-23 Thread AGrobe2555
You'll need to resuspend your pellet in a limited volume, do a cell count (using a hemocytometer, etc) and dilute the original suspension to the desired cell#/ul concentration with an appropriate diluent. Albert Albert C. Grobe, PhD Tissue Engineering Lab International Heart Institute of Mo

[Histonet] Histology Lab Survey Invitation

2008-10-23 Thread Smith, Brett
I represent a medical device company researching a concern in the histology lab and would like to invite you all to participate in a short, 2 - 4 minute survey. This survey will help verify a potential product need or will help prove that the need for a product does not exist. Please click on the

[Histonet] Histotechnology Society of Delaware - Fall Open Meeting and Hayride/Bonfire

2008-10-23 Thread Kristen Yaros
This is a reminder to all Delaware members and non-members that we will be holding our open meeting and Hayride/Bonfire at Carousel Park this Saturday, October, 25th from 3:00 to 6:00 pm. 3:00 Food and Open Meeting 4:00 Hayride 5:00 Bonfire and Smore's HSD is providing the main food. Everyone is

[Histonet] Tracy Bergeron is out of the office.

2008-10-23 Thread Tracy Bergeron
I will be out of the office starting 10/22/2008 and will not return until 10/27/2008. I will respond to your message when I return. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/h

Re: [Histonet] Ab validation

2008-10-23 Thread renafail
Absolutely Rena -- Original message from [EMAIL PROTECTED]: -- > Would you agree that if you change the pretreatment vessel you would have > to revalidate all your antibodies? Or if you change your pretreatment > solution to an all in one solution that deparaffinizes

RE: [Histonet] Ab validation

2008-10-23 Thread Sebree Linda A.
Yes, we would reevaluate and get the new procedures signed-off by our director. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED]

Re: [Histonet] Ab validation

2008-10-23 Thread pkromund
Would you agree that if you change the pretreatment vessel you would have to revalidate all your antibodies? Or if you change your pretreatment solution to an all in one solution that deparaffinizes & does HIER that you would also have to revalidate all your antibodies. Pam

[Histonet] (no subject)

2008-10-23 Thread Sadushe Loxha
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RE: Technical vs. Professional Re: [Histonet] 88305 pod lab question

2008-10-23 Thread Weems, Joyce
http://www.google.com/search?hl=en&q=pod+labs&aq=f&oq= Check out the info at this google search. It will eventually be stopped, I'm sure, but not before a lot of money is wasted setting up histo labs where they don't need to be and pathologists losing technical fees with global billing, ghhh..

RE: Technical vs. Professional Re: [Histonet] 88305 pod lab question

2008-10-23 Thread Blazek, Linda
What is the definition of a Pod Lab? -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Weems, Joyce Sent: Thursday, October 23, 2008 11:38 AM To: Mark Tarango; Cheryl Cc: histonet@lists.utsouthwestern.edu Subject: RE: Technical vs. Professional Re: [Histo

RE: Technical vs. Professional Re: [Histonet] 88305 pod lab question

2008-10-23 Thread Weems, Joyce
Which is why everyone is trying to scramble the gray areas to make it work for them, including urologists who are taking money away from the pathologists. We all need to work to correct this process before it gets totally out of hand. For some reason beyond me, our politicians cannot understand thi

Re: Technical vs. Professional Re: [Histonet] 88305 pod lab question

2008-10-23 Thread Mark Tarango
Hi Cheryl, I looked up 88305-TC and it looks like they pay around $60 for this code. 88305-26(professional component) only pays around $40 depending on locality. They pay more to make the slide than read it. Mark On 10/23/08, Cheryl <[EMAIL PROTECTED]> wrote: > Can anyone clear this up with ce

Re: [Histonet] 88305 pod lab question

2008-10-23 Thread Mark Tarango
Well just like Roxanne I quit my job over this. I brought up the way he charges in the job interview and he claimed that he had letters from lawyers that said it was okay and that he DIDN'T actually bill medicare for 88305-TC, but only insurance companies. When I started working there in billing

[Histonet] Celestine Blue

2008-10-23 Thread Scott Hooten
I was wondering if anyone knew of any place to buy pre-made celestine blue. Right now we have to buy the separate chemicals and mix up our own, so if anyone knows of a place that sells pre-made celestine blue I would appreciate it. Thanks. Scott R. Hooten Histology Technician MED Institute 1 Ge

RE: Technical vs. Professional Re: [Histonet] 88305 pod lab question

2008-10-23 Thread Cheryl
Can anyone clear this up with certainty?  We aren't billing MC/MD so Marla may be totally correct on this.  I read the full code book as published last year and didn't come away with quite this same distinction and I would really like to know!   Cheryl   Full Staff Inc. Staffing Healthcare Profe

Re: [Histonet] TUNEL on mouse bone marrow

2008-10-23 Thread anh2006
I had the same experience and thought it was most likely due to the recombination that occurs in the BM during lymphocyte maturation - so the reagents are giving false positives. So instead I use cleaved caspase 3 IHC. --Original Message-- From: FU,DONGTAO Sender: [EMAIL PROTECTED] To:

Re: [Histonet] RE: [IHCRG] HIER

2008-10-23 Thread anh2006
I too am all about the steamer. We use a veggie steamer (only costs about 10-15 dollars) and heat the solution to 95 deg C for a 20 min retrieval. Works great. -Original Message- From: Patsy Ruegg <[EMAIL PROTECTED]> Date: Thu, 23 Oct 2008 07:15:47 To: <[EMAIL PROTECTED]>; <[EMAIL PROTE

RE: Technical vs. Professional Re: [Histonet] 88305 pod lab question

2008-10-23 Thread Marla Thomas
If I am not mistaken, for MD/MC it is the unit performing the technical who can bill for the global component. If you only do the professional component you cannot bill for the technical. That is why with most POD Labs they must provide the technical themselves in order to bill globally. Many of

Technical vs. Professional Re: [Histonet] 88305 pod lab question

2008-10-23 Thread Cheryl
Hi Mark--   The technical and professional on this code are generally bundled.  For most insurances as well as M/M it can't be split it to be reimbursed (and it HAS to be billed by the party performing the professional is my understanding after just working on this issue a few months ago), Thus

[Histonet] Manual processing of radioactive mouse brains

2008-10-23 Thread Jamie E Erickson
Hello histoneters, I have a question for anyone out in histoland that manually processes tissue. I have a study that will be using radioactive I125 mouse brain tissue and I don't want to process these in our Lecia TP 1050 auto processor for risk of contaminatin

[Histonet] TUNEL on mouse bone marrow

2008-10-23 Thread FU,DONGTAO
Hi, all We met a problem with TUNEL staining on mouse BM(10% NBF fixed paraffin). The protocol we used works very well with other tissues, however, it gave us high background on bone marrow. Can anyone give us some suggestions on TUNEL staining using bone marrow? Many thanks, Ann Dongtao

[Histonet] RE: [IHCRG] HIER

2008-10-23 Thread Patsy Ruegg
I have used many different hier methods over the years and have special considerations because of my altitude. We thought that the pressure cooker would solve all the problems relating to altitude because it is pressurized but it did not. There must be some boiling going on sometime because I oft

Re: [Histonet] 88305 pod lab question

2008-10-23 Thread godsgalnow
If the histology is outsourced the technical portion cannot be charged.? Although, I know some pod labs, that have found loopholes to this--that is whay I no longer work for one. Roxanne -Original Message- From: Mark Tarango <[EMAIL PROTECTED]> To: histonet Sent: Thu, 23 Oct 2008 12:5

[Histonet] need help for cultured cells

2008-10-23 Thread Nejat Yilmaz
Dear Netters, How can I set 50.000 cells in 1 ul suspension from a cultured cell pellet? I need your kindly help. Best regards... Dr. Necat Yilmaz ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman

[Histonet] peristaltic pump setup/storage of frozen rat brain

2008-10-23 Thread Michael Patric
Hello Everyone, A colleague recommended this group to me. I hope you will be able to help me with three questions that I have: 1) I am trying to setup a Masterflex L/S peristaltic economy drive pump system to assist in the perfusion of adult rats. We are interested in collecting the brain f