Dear Tahseen
1) You can contact your microtome vendor for blade cover/shutter
2) Be careful while sectioning, offcourse this come by experience.
3)Using glove will invite more trouble and may not be handy.
Regards
Chandrashekar BG
- Forwarded by BC Girish/PreClinSafety/DR-India/DRL/IN o
Thanks Carl for your valuable suggestions on anti- phospho-Histone H3
antibody.
But i will be glad if someone can share the staining protocol for Toluidine
blue and Crystal violet to demonstrate mitotic figures.
Regards
Girish
Disclaimer
This message contains legally privileged and/or confid
Hi all
My lab is interested in purchasing an automated immunohistochemistry stainer
and I would like some feedback on the new stainers that are out there. Is
anyone using the Celerus Wave or Biocare's Intellepath stainer. Any
opinions on either of these stainers, good and bad is greatly apprecia
Hi Ian,
Dermestid beetles are definitely the least smelly option
(although they are not perfect). You'll find that there will usually be
a residual smell in the short term. They can, however, damage small or
delicate bones and the best way to deal with these is by bacterial
macerat
Factor VIII (factor 8, anti-hemophilic factor) is present in vascular
endothelium and, and along with CD34 is used as an immunohistochemical
marker for small blood vessels. Factor VIIIa is the activated form of
this clotting factor.
von Willebrand factor is an entirely different molecule, also in
Hi Gudrun,
Any antibody I have used for vWF detects the complex (DAKO's A0082 pAb for
example) ...
I believe the Factor VIIIa comes into play once cleaved as you mention below.
Is this something you need to specifically be concerned with? I thought
you/your PI just wanted a good pan endothelia
What detection system are you using?
Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. #215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
[EMAIL PROTECTED]
www.ihctech.net
www.ihcrg.org
-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of soofia
s
I would think they are alright if they have been kept lyophilized at 4dc, I
would certainly try them. Once you reconstitute them, freeze small aliquots
for future use unless they are hrp conjugated.
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. #215
Aurora, CO 80045
720-859-4060
fa
Andrea,
I agree with your approaches to keep tissue IgG from binding to the anti
human secondary, I especially like the fitc conjugation of the primary then
use rab anti-fitc, then rab labeled polymer. The reason I avoid SAB is
because of endogenous biotin issues. I prefer hrp or ap labeled poly
Very true Patsy, Amos' proposed scheme will not eliminate the problem of
human-on-human.
However, I do not see how using extra host secondary serum will block the
anti-human from binding the endogenous IgG in the tissue. And using human serum
will just make your background worse or eliminate st
The only problem I see with this is similar to running an anti mouse ab on
mouse tissue. The secondary link anti human IgG will bind to all IgG in the
human tissue, not just the primary antibody, so you would need to use serum
blocks to prevent that. I would use 10% serum from the host of the
sec
Diastase powder will dissolve in any water, but requires some calcium ions
to function. Thus it will not perform in distilled water, but will perform
well using plain tap water. Also, the diastase powder from sigma will work
well if only a very small amount of the powder is used. We always used jus
Hilda:
Under separate cover I am sending an article I wrote on this very same subject.
René J.
--- On Sat, 10/25/08, shazana hilda <[EMAIL PROTECTED]> wrote:
From: shazana hilda <[EMAIL PROTECTED]>
Subject: [Histonet] IHC scoring system
To: histonet@lists.utsouthwestern.edu
Date: Saturday, Octobe
Use the "Allred" scoring system (see Mod Pathol 1998;11(2):155-168).
Richard
Richard W. Cartun, Ph.D.
Director, Histology & Immunopathology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596
(860) 545-0174 Fax
>>> shazana hilda <[EMAIL P
Dear Tyrone (and others),
I'm routinely and happily using egg white powder (really raw avidin) and
biotin (by Sigma) to block endogenous biotin, as suggested earlier on
Histonet.
Before primary ab (and perhaps also the serum block, doesn't really matter),
incubate slides for 15 min in 10% (w
Many thanks to all that answered my question, but I am still in doubt.
This is from Wikipedia:
"Von Willebrand factor is not an enzyme and therefore has no catalytic
activity. Its primary function is binding to other proteins, particularly
Factor VIII and it is important in platelet adhesion t
16 matches
Mail list logo
