[Histonet] Microtome calibration

Dear all Is anyone practising microtome calibration for thickness of sections cut? Our GLP auditors are frequently asking for it. They also suggest that automatic tissue processor time in each reagent should be calibrated. any comments Regards Dr Girish India Disclaimer This message

[Histonet] PIN-4 controls

Happy Halloween! Does anyone know of a good PIN-4 control? We are currently using in-house tissue but are finding a lot of variance with the stainability. If anyone knows of a good source I would greatly appreciate it. Thanks in advance, Sharon Campbell, HTL(ASCP)CM, BSBM Histology

[Histonet] pronase vs. proteinase-k

Are they the same ? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax This e-mail and any accompanying attachments may be privileged, confidential, contain

[Histonet] anti-mouse ferritin

Hi,   Any recommedations for anti-mouse ferritin antibodies (looking to stain FFPE mouse tissue).   Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

Re: [Histonet] Microtome calibration

Dear Dr. Girish, I agree with rene, if the qualitiy of the cuts is fine it doesn't matter if they are 1µm or 2.3µm thick. But I'm highly interested in how your GLP auditors want to calibrate the thickness of the section cuts. Are you supposed to measure the slides after cutting?! Before or after

Re: [Histonet] Microtome calibration

Frauke: That calibration is ussually done by measuring the advance mechanism of the block holder and how many µm the block moves towards the blade, but that is rubbish. René J. --- On Fri, 10/31/08, Dr. med. Frauke Neff [EMAIL PROTECTED] wrote: From: Dr. med. Frauke Neff [EMAIL PROTECTED]

RE: [Histonet] Microtome calibration

We have a GLP person who is not familiar with Histology at all. We have had to educate her about these issues and may other things. Calibration finally came down to having a PM on the units every year. After she understood all the things we do with a section during the cutting phase and pick up

[Histonet] Polyclonal Proteolipid Protein Ab?

I have been (unsuccessfully) trying to find a polyclonal antibody against human proteolipid protein (stains myelin) that will work with immunofluorescence on FFPE sections. I have also tried some myelin basic protein with no luck. Has anyone found any in the past that have worked?? Thanks!

[Histonet] Formalin and Xylene Monitoring Badges

Happy Halloween... I'm looking for a source of monitoring badges for formalin and xylene, both the 8 hour TWA and the STEL. Thank you, Sandy Sandra Cheasty Histology Supervisor UW-Madison School of Veterinary Medicine 608 263-1680

Re: [Histonet] Formalin and Xylene Monitoring Badges

ASSAY TECHNOLOGY 1252 Quarry Lane Pleasanton, CA 94566 1-800-833-1258 Sandra Cheasty [EMAIL PROTECTED] 10/31/2008 08:51 Happy Halloween... I'm looking for a source of monitoring badges for formalin and xylene, both the 8 hour TWA and the STEL. Thank you, Sandy Sandra Cheasty Histology

[Histonet] RE: Formalin and Xylene Monitoring Badges

Try Mercedes Medical. http://www.mercedesmedical.com/ Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: [EMAIL PROTECTED] -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED]

RE: [Histonet] Formalin and Xylene Monitoring Badges

Morphix Technologies www.morphtec.com 757-431-2260 -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Kathleen Boozer Sent: Friday, October 31, 2008 11:22 AM To: histonet@lists.utsouthwestern.edu; Sandra Cheasty Subject: Re: [Histonet] Formalin and Xylene

[Histonet] Re: bone marrow biopsies

Cindi Robinson replied to me offline, saying some of the things Vinnie della Speranza also said. I wasn't aware that hydrochloric acid decalcification (I assume that regular brand-name Decal is HCl) with prompt timing degraded IHC - something I guess that every lab has to determine for themselves.