RE: [Histonet] Advanced Histo training

2008-11-22 Thread Lee Peggy Wenk
I don't know this person, so I'll answer in general. I get a lot of these types of requests at my school. Most seem to be from the Middle East or Africa. When emailing with them, it mostly appears that they just want to find a quicker way to get into the US. Realize that in most cases, if they

[Histonet] ISH question

2008-11-22 Thread Gudrun Lang
Hi listmembers, In our lab the unstained IHC-slides are thrown away. Now one slide of each case should be stored in an adequate manner to perform an insitu hybridization if required in the future. I want to store it unstained but deparaffinized and coverslipped. So that it can be filed with the

[Histonet] Re: Colon Cancer

2008-11-22 Thread Shelly Coker
I work with many GI biopsies and occassionally we send off slides for testing for MSI (Microsattelite Instability).  This is a test for Hereditary Nonpolyposis Colorectal Cancer (HNPCC), also known as Lynch Sydrome.  The IHC tests for normal gene expression. You can google HNPCC or MSI and find

Re: [Histonet] RE: Dako and Leica immunostainers

2008-11-22 Thread Larry Woody
Dako doesn't even manufacture an IHC stainer unless you count the Eridan which was a failure. The instrument that they distribute is manufactured by Lab Vision/ now Thermo Shandon and hardly a dinosaur. If a person doesn't know the theory behind doing IHC or can't perform IHC on the bench then

Re: [Histonet] RE: Dako and Leica immunostainers

2008-11-22 Thread John Steel
The Dako Eridan was never released, thus is a moot point, and agree that the current open platform Dako offers is very flexible for a variety of uses - with a caveat.  Different instruments require different levels of IHC experience, IHC confort level, and who pre-screens stained slides prior

[Histonet] Test only #1. Don't bother to read or reply.

2008-11-22 Thread John Kiernan
Some of my recent posts to the Histonet Listserver have bee garbled, although other emails that I send are OK. These few lines are sent as plain text to see if they look OK when distributed to the List. This is a second paragraph. John K. = = =

[Histonet] Test message #2. Don't bother to read it.

2008-11-22 Thread John Kiernan
Some of my recent posts to the Histonet Listserver have bee garbled, although other emails that I send are OK. These few lines are sent as rich (HTML) text (HTML) to see if they look OK when distributed to the List. This is a second paragraph. John K. = = =

Re: [Histonet] Stain for Cellulose

2008-11-22 Thread John Kiernan
Cellulose isn't a mammalian tisssue element, but there's usually some to be seen in the lumen of the alimentary tract, especially stomach and colon, of rats, mice etc. Cellulose is PAS-positive. It's also birefringent (if you have a microscope with polarizing filters), but mostly it shows up