Re: [Histonet] Buffy coat preparation for immuno

2008-11-30 Thread John Kiernan
Why go to all the trouble of embedding and sectioning a buffy coat specimen? What's wrong with diluting it in saline and then making smears or (better, if you have the equipment) cyto-centrifuge preparations? You'll get lots of slides. They can be fixed in methanol and the WBC stained with any c

[Histonet] Buffy coat preparation for immuno

2008-11-30 Thread Stephen KumJew
Hi from Down Under I was wondering if anyone had a method for preparing buffy coats for immuno. What we have done so far 1) is spun down whole blood, extracted the buffy coat and made a thrombin/plasma clot of the extracted buffy coat (minus RBCs). The clot was post fixed overnight in 10

[Histonet] Job posting for IHC tech at Vitro Molecular Laboratories

2008-11-30 Thread ancillarypath
Dear colleagues, Vitro Molecular Laboratories is in a need for an additional histotech with expertise in immunohistochemistry. The laboratory is based in South FL. The position is a full-time position, with potential for growth. Knowledge in basic and advanced IHC is required, particularl