I always prepared PFA as at least an 8% solution so that dilution with buffer
would give me the final working %. The protocol was to add the PFA powder to
water at 50C and then add 10N NaOH dropwise until the solution cleared. After
cooling to RT, the stock PFA solution was filtered to remove
During time, besides the re-polymerization, methanal (= formaldehyde) turns
into methanoic (acid) and the buffer salts prevent that acidification.
René J.
--- On Sat, 12/6/08, [EMAIL PROTECTED] <[EMAIL PROTECTED]> wrote:
From: [EMAIL PROTECTED] <[EMAIL PROTECTED]>
Subject: [Histonet] divalent io
You were taught correctly. And the socalled "degradation" is re-polymerization
as you wrote. The only thing is that you should add buffer salts bettwen than
PBS.
René J.
--- On Sat, 12/6/08, [EMAIL PROTECTED] <[EMAIL PROTECTED]> wrote:
From: [EMAIL PROTECTED] <[EMAIL PROTECTED]>
Subject: [Histo
Hello,
Can anyone tell me their experiences with the Hologic's Cellient Cell Block
System?
I appreciate any thoughts and comments about the system you have.
Paula Lucas
Lab Manager
Bio-Path Medical Group
Fountain Valley, CA
___
Histonet mailing list
Hi,
What is the function of divalent ions in fix?
It's been suggested to me that by using Dulbeccos PBS to make formaldehyde
fix from PFA will better preserve tissues, or at least better preserve
proteins.
Eric Schmidt
University of Calgary
Medical Sciences
Hi,
So then what is the best way to prepare formaldehyde fixative from PFA?
The way I have been taught, which differs from what I have read, is to
dissolve 4% into ddH2O at room temperature. After that one could add
PBS or buffer.
I've also been taught that too much heat during preparat
Interesting question.
Post fixation is sometimes carried out to enhance staining. I the old days
we fixed in formalin, then in mercury. If you fixed in mercury from the
start then the tissue went hard whilst you could leave tissue in formalin
for ages; you can therefore control the exposure ti
