[Histonet] (no subject)

2008-12-18 Thread Diana McCaig
What should the pH range be for Scott's Tap Water? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Which Blocking reagent?

2008-12-18 Thread Kimberly Tuttle
Im trying to work up a few IHC stains, but Im still learning too. Synaptopodin using a kidney control and Cleaved caspase 3 on tonsil. Im getting a lot of background but there are so many reagents out there I dont know which to try or whats the difference between them. Im using the Dako

[Histonet] Fixing and IHC on PEG hydrgoels

2008-12-18 Thread Stephens, Elizabeth Humes
Dear Colleagues, I was wondering if anyone has had any experience with fixing and performing immunohistochemistry on cells seeded onto PEG hydrogels. These are very hydrophilic gels and I have heard that, even with a very short fixation time, fixation causes any cells that were growing on the

[Histonet] Capillary counting

2008-12-18 Thread Nejat Yilmaz
Hi All, We need a protocol demonstrating capillaries in sceletal muscle tissue other than immunohistochemistry. We're planning to compare number of capillaries between different experiment groups for evaluating angiogenesis. Is there any special stain to suggest for this purpose? Thanks in

[Histonet] PowerPath

2008-12-18 Thread Linke, Noelle
Hi everyone, I am new to Power Path and I have a question I was hoping someone could answer. Is there a way to pull metrics from it (slide totals, stains, etc)?? Any help would be appreciated! Thank you! Noelle Noёlle Linke M.S., HTL(ASCP)QIHC Manager, Histology Services Department of

Re: [Histonet] Capillary counting

2008-12-18 Thread Rene J Buesa
Nejat: There are several stains for capillaries: 1- Campbell and Alexander (tested by Mallory,1938): black agsinst colorless bbackground. 2- Doherty, Suk and Alexander, 1938: differential staining of blood in capillaries. 3- Pickworth, 1934: blood vessels (capillaries) in thick sections of

[Histonet] Mature endothelium marker

2008-12-18 Thread Karen Lapanowski
Hello, Does anyone know of a mature endothelium marker other than Factor VIII that can be used in rat brain paraffin sections? Thanks, Karen Lapanowski Henry Ford Health System Radiation Oncology 3065 E R 313-916-9386

Re: [Histonet] Mature endothelium marker

2008-12-18 Thread Rene J Buesa
Use Ulex europaeus lectins. René J. --- On Thu, 12/18/08, Karen Lapanowski klapa...@hfhs.org wrote: From: Karen Lapanowski klapa...@hfhs.org Subject: [Histonet] Mature endothelium marker To: histonet@lists.utsouthwestern.edu Date: Thursday, December 18, 2008, 3:53 PM Hello, Does anyone know of

[Histonet] dako artisan gram and afb

2008-12-18 Thread Carrie Disbrow
Happy Holidays to all. W ea re using the Dako Artisan stainer and have had problems lately with the AFB and Gram staining inconsistently from day to day. We have had the machine services and the PM done and the problem has returned. Has anyone had the same problem and what did you do? Thanks