RE: [Histonet] used hacker bright cryostat...

Tom, We have a few used Bright OTF/AS models available but they are 230v 50 htz so will not suit your region in you are in the USA, if so hopefully one of our distributors will see your posting. Please take into account that it is very, very favorable to purchase from the UK at present. Best

RE: [Histonet] Microtome Cutting Safety

I use my fingers too, so no help there. But an automated microtome is great because it frees both hands to handle a ribbon as it is coming off the blade. It is easy to get careless if you use a foot pedal but the one time I have been seriously cut it was with a manual microtome so I guess the

[Histonet] Outside Lab

Hi histonetters, Hope everyone is having a good week so far... Our hospital receives specimens from a Urology group on a daily basis. Our PA grosses the specimens here; us histotechs work our magic, and the hospital pathologists take the slides directly to the Urology office to read them

RE: [Histonet] Outside Lab

Go to http://www.cms.hhs.gov/center/asc.asp for more information about Ambulatory Surgical Centers (ASC's) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Senn, Amy R Sent: Wednesday, January 07, 2009 8:28

RE: [Histonet] Outside Lab

Is the histo lab in the urology office CAP certified? Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] IHC Doublestain

I am supposed to try a doublestain using the DAKO autostainer where one of the antibodies requires a Proteinase K pretreatment and the other antibody requires antigen retrieval with heat. Should I do both, or will this not work at all? Thank you Kimberly C. Tuttle HT (ASCP) Pathology

Re: [Histonet] Microtome Cutting Safety

Donna Leica makes a microtome series RM2200 that allows you to cut sections with the knife guard up. You will be able to decide whether you would like a rotary microtome or a automated microtome. I see that you like the Leica CM3050. The comparable microtome for paraffin cutting is the RM2255.

RE: [Histonet] Outside Lab

Why does the hospital pathologist go to the Urology to read and sign out the cases? So if I am understanding this correctly, your hospital is right now getting the tech part of the specimen for processing. Is your pathologist part of the hospital or are they their own group? The urology group will

Re: [Histonet] Outside Lab

The pathologists are probably going to the urology group to read because CLIA certification is LOCATION specific. The urology group may already have CLIA cert. and is splitting the interpretation charges with the pathologist. By adding its own lab, they will be able to capture both tech and

[Histonet] RE: equation problem PLEASE help

Hi Eva. Here’s how I would do it. (1 mole/1064 grams)*(.001 gram/ml)= 9.4 X 10^-7 mole/ml Concentration 1*Volume 1= Concentration 2 * Volume 2, or C1V1=C2V2 C1= 9.4 X 10^-7 mole/ml C2= 10X 10^-9 mole/ml V2= 2 ml Solving for V1... V1= (C2V2)/C1 V1= (10 X 10^-9

Re: [Histonet] Cryostat safety question

Hi Andrea, It's great to hear that you are happy with your cryostat and, hopefully, I'll be able to help you with an answer to your question. Leica sells a small device called a Thermal Block that is designed to help you remove the specimen from the chuck without causing it to melt. The catalog

RE: [Histonet] Cryostat safety question

Andrea: I work in a Mohs clinic where all we cut is frozen skin sections. Needless to say, we don't have 50 chucks laying around... In the morning before clinic starts we put a layer of freezing medium on chucks and put them in the cryostat to freeze. When we get specimens, we add another drop

Re: [Histonet] Cryostat safety question

I have the same cyostat, but it doesn't matter which one you use for removing tissue from the chuck is a separate issue. Others may beg to differ from my technic, but I remove the block from the cryostat and set it on a paper towel for a couple of mintes. I do not thaw tissue, just soften it. I

RE: [Histonet] Microtome Cutting Safety

I guess I wasn't totally clear in my earlier reply. I use my bare fingers but I also use a camel hair brush as Beth states. I direct the brush with my right hand and pick up the first part of the ribbon with my left. The brush is used to hold the last part of the ribbon to aid in maneuvering

Re: [Histonet] equation problem PLEASE help

Hello Eva Put simply 1nmol is 10-9 moles Therefore 10nmol is 10-8 moles 1 mole = 1,064g Therefore 10-8 moles = 0.1064gor 0.01064mg Therefore your working solution of 10nmol/ml = 0.01064mg/ml Your stock solution is 1mg/ml Therefore your dilution factor is 1.0

Re: [Histonet] equation problem PLEASE help

Hello Eva Unfortunately some of the script did not transfer in the original mail and 10-9 should read 10 to the minus 9 as the superscript was lost. The same for 10-8. Also there should have been a division line between 1.0 and 0.01064 in the calculation. I hope this helps. regards