James,
You did not say how long you were fixing these somewhat large embryos? Nor
did you give the processing schedule in detail, time/solvent/clearing
agent/paraffin? Please provide this info, thanks!
If the tissues are soft in the center, over dehydration is probably not the
problem. It
If your problem is sectioning, probably the problem is due to infiltration and
the cause of the defective infiltration could be fixation, dehydration and/or
clearing.
Without knowing your protocol it is difficult to try to try to find out the
cause.
René J.
--- On Fri, 1/9/09, James Dooley wro
Hello All,
I
have been having trouble cutting whole embryo sections on E16 mice. I
think the problem is with the fixation process. I am currently using a
modified Carnoy's fixation (60% ETOH, 30% formaldehyde, 10% Glacial
acetic acid). I would like to use this fixation as I have optimized my
antib
Roger:
To rely in the "basic" quality of a tap water is a risky and inconsistent
approach because not all tap waters are "created equal" and they could even
vary along the year.
My advise is that you use a basic solution, either a diluted (1-2%) ammonium
hydroxyde or a 1-2% lithium carbonate sol
Mayer's hemalum is a nuclear selective progressive formula so the staining
is largely confined to nuclei. If the pathologists want more blue tinge in
other parts of the tissue, then you should change the hemalum, or simply
increase the hematoxylin content in the solution. Cole's does stain dar
We are currently seeking a flow cytometry technologist for our pathology
laboratory in Lakeland, Florida. Experience and appropriate state licensure is
required. If interested, send resume via e-mail or web site,
www.micropathlabs.com. No recruiters please.
Thank you,
Sheila Haas
Laboratory Supe
Some tap waters blue hematoxylin very well, some not so well. I avoid the
issue of differences in tap waters by blueing in 1.2% aqueous lithium carbonate
(almost a saturated solution).
- Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University School of Podiatric Medicine
Mi
Hello All,
TGIF
I'm trying to help out one of my veterinarian pathologist in getting some
information on how to increase the bluing of the H&E staining. Presently we
are using pre made Mayers Hematoxylin from Sigma with an automated schedule as
follows:
1. Drying 45min
2. citrisolve 3
Hi all, just wonder any one has the protocol for Feulgen Nucleal Reaction on
thick brain sections, such as 20-40 um>
I have one protocol refering to:
http://stainsfile.info/StainsFile/stain/schiff/feulgen.htm
2009-01-09
TF
___
Histonet mailing
The chick hindlimbs are removed and washed in chick ringer's solution (a salt
solution to wash off and debris) then fresh frozen with cryo-m-bed. On the
sections we fix with 2% PFA for 10mins, but the problem is arising before the
sectioning. I am sure that the stain should be stronger as the st
We used to do a stain for uric acid, but we've discontinued that and now
the pathologists only scrape it & put it on a slide with a coverslip on
top to determine if there are urate crystals. My question is, is there
a point to doing a special processing of the tissue from 100% to
paraffin, if when
Dear Anjan Kumar,IHC triple staining based on three different chromogens is
possible with using various combinations of DAB (HRP), Vector VIP (HRP), Liquid
Permanent Red (AP), Vector Blue, X-gal (beta-GAL). As long as the primary
antibodies of interest do no show co-localization, you may end up
you know that TBS works for bluing any hematoxylin.
Gene
-Original Message-
From: Kelly Boyd
To: histonet
Sent: Thu, 8 Jan 2009 2:46 pm
Subject: Re: [Histonet] Hematoxylin Counterstain for IHC slides
Biocare's Cat Hematoxylin is really good. One real nice feature is that you
blue
Does anyone know of a supplier where I can purchase slides with calcified
tissue, preferably aortic tissue?
I want to evaluate the degree of vascular calcification in an in house
animal model and identify the most suitable staining for this model
(alizarin, vonKossa or MacNeal).
In order to imp
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