[Histonet] Does anyone know why skin sections do not seem to take up haematoxylin as well as other tissues. The chromatin detail is not as good as with other tissues.

Does anyone know why skin sections do not seem to take up haematoxylin as well as other tissues. The chromatin detail is not as good as with other tissues. ** This email and any files transmitted with it are confidential and

[Histonet] H+E Skin Staining

I am interested in the reasons behind the lack of chromatin detail in skin sections. Although this is not always a problem it is a concern. Could the problem be the processing? or are there other factors related to skin? **

[Histonet] Microm HM325 users

Hello Histonetters! Is anyone using a Microm HM325 microtome? Please contact me if you want to share some tricks and useful hints, concerning sectioning to me :) I don't have a question in particular, just want to see if anyone is doing sth like cooling the blade or so. Bye, V. Neubert

[Histonet] DAB

Greetings I recall that years ago Biomeda had a product called Stable DAB. It was a ready-to-use product that was stored in the freezer. Does anyone know if it is still being produced and whom I should contact? William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10

[Histonet] RE: DAB

There was a discussion about the Stable DAB on the histonet a few weeks ago. I would suggest you search the archieves of the histonet. The company that has this product was listed at that time. Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic

Re: [Histonet] DAB

Greetings! The research scientist of Biomeda has founded ImmunoBioScience Corp., therefore offering a direct replacement of Biomeda products. Please visit www.ImmunoBioScience.com Thank you. Best regards, Anita -Original Message- From: O'Donnell, Bill

[Histonet] curling on cryostat brain sections and other issues

Hi there, I am having problems with my brain sections (15 um - flash-frozen) curling up once I remove the antiroll plate on my cryostat... does anyone know how i can prevent this? Also - does anybody have a quick guide on what to improve while cutting, based on the damage seen on the

Re: [Histonet] curling on cryostat brain sections and other issues

I am having problems with my brain sections (15 um - flash-frozen) curling up once I remove the antiroll plate on my cryostat... I cut many of the same on a regular basis, and I find that letting them sit for a a few moments (~10 seconds) helps keep them from curling, at least long enough

Re: [Histonet] curling on cryostat brain sections and other issues

er, sit for a few moments under the anti-curling plate, that is... Peter Carroll wrote: I am having problems with my brain sections (15 um - flash-frozen) curling up once I remove the antiroll plate on my cryostat... I cut many of the same on a regular basis, and I find that letting them

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Re: [Histonet] curling on cryostat brain sections and other issues

I make 8um-thick sections of various tissues and sometimes they do curl. I do not use an anti-roll plate. So what I do while making the section is to cut just enough of the section so that it is attached by a very small amount to the top of the block, (so that most of the block has gone down

[Histonet] Grossing

CAP is OK with their guidelines ( BUT is CLIA on line with the Processing (Low Complexity?) vs Grossing (High Complexity?). If CLIA is not on board with processing (dumb naming) being low complexity and grossing being higher complexity your lab may be in violation of CLIA Guidelines.

[Histonet] re:curling on cryostat brain sections and other issues

At the temperture just after what I call the crumple stage, when the tissue and medium are stiff enough to form under the blade in a complete section, the section will lie flat with out shattering. If your brush is clean you can cut multiple in a row like ribbons. This is in the - 16 to -18C

[Histonet] Florida Histo Openings

Good morning. We are a Pathology Lab in Pinellas Park, Florida. We're looking to add to our team! Candidates should be an HTL or HT (ASCP) or equivalent, have a valid driver's license and proof of insurability. Primary responsibilities include on site frozen sections including mobile

[Histonet] succinate dehydrogenase protocol

Dear Colleagues I should detect /stain Succinate dehydrogenase in mouse muscle non-fixed frozen sections. Could someone please share detailed protocol and sources of the reagents. Does it exist any   kit for detection? I performed some on-line search but did not find a sufficient information.

[Histonet] euchromatin and heterochromatin

Hi dear histonetters, I have a question about chromatin and its stainability. Heterochromatin is said to be the inactive part (perhaps hypermethylated) and the only part of the chromatin, that can be stained. Euchromatin on the other side is the active part and can't be stained. The question is:

[Histonet] re:Hematoxylin Staining of Skin

My first question is in what region of skin is the staining poor? The fibroblasts of the dermis should stain really well with HE = as should the cells of the stratum germinativum. From there out nucle ar staining gets worse and worse. I attribute it to two things, and

RE: [Histonet] Does anyone know why skin sections do not seem to take up haematoxylin as well as other tissues. The chromatin detail is not as good as with other tissues.

Sarah, Skin surface tends to dry out as well as the epithelial cells lose their nuclei as they migrate to the surface. The surface can also dry out after the application of antiseptics etc prior to biopsy. AND there is often the issue of the biopsy being allowed to dry prior to fixation. This

RE: [Histonet] RE: DAB

I have found the following method easy to use. The DAB Concentrate is aliquoted in small volumes and frozen. It is quite stable for at least a year. The following method is also used by our Haematology department for their myeloperoxidase staining: Peroxidase Peroxidase catalyses the transfer

Re: Re: [Histonet] BrdU immunostain on rat brain section

Abcam monoclonal rat-anti BrdU http://www.abcam.com/BrdU-antibody-BU1-75-ICR1-ab6326.html Just compare to other Abcam anti-BrdU, it has most citations at least. 2009-03-18 TF 发件人： Amy Lee 发送时间： 2009-03-18 07:14:56 收件人： tifei 抄送： 主题： Re: [Histonet] BrdU immunostain on rat brain

Re: [Histonet] succinate dehydrogenase protocol

We stain for SDH in fresh frozen mouse skeletal muscle using a kit from Sigma. Galina Deyneko galinadeyn...@yahoo.com Sent by: histonet-boun...@lists.utsouthwestern.edu 03/17/2009 12:45 PM Please respond to galinadeyn...@yahoo.com To histonet@lists.utsouthwestern.edu cc Subject

[Histonet] Cutting fresh-frozen brains from 1 week old rat pups

Hi all, Currently I am working with brains from 7 day old rat pups, that undergo an hypoxic-ischemic injury (Levine/Vannucci technique). These brains are unfixed, frozen in isopentane and cut at 20um on a croystat. These brains are not cutting very well compared to an adult brain (potenially