RE: [Histonet] question regarding rules for disposal of slides cassettes

Hi Jenee: You need to check the histonet archieves. For the last two weeks there has been an on going conversation in regard to disposal of microscopic slides. Since I am in a COR lab most of my slides are sent to the PI's that order the slides therefore I do not have to deal with this as of

[Histonet] OT: Books

Gosh - my retirement fund just appeared... a Sheehan for $2500? Wow! I have one here (2nd edition) but not mine to sell - besides, I use it. I do have a 3rd edition Lillie Histopathologic Technic and Practical Histochemistry (1965), pre-autographed by me when I bought it that I'd sell for the

[Histonet] RE: OT: Books

Make sure you get enough to have visitors! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lbla...@digestivespecialists.com -Original Message- From:

[Histonet] Quality Improvement

I'm curious as to what others do, if anything, to document corrective actions or improvements for HE staining and/or tissue processing. For example, I made changes to my biopsy processing program and to my breast processing program which helped improve the cutting quality of both types of tissue.

RE: [Histonet] RE: OT: Books

So what does an Anne Preece go for? Can I retire too? I've got one of those Sheehan's too. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From:

[Histonet] More about distilled water

I would like to add to the cooments of others concerning distilled water and deionised water: 1) I think most histology labs use deionised water as it is cheaper and easier? to obtain. 2) However, even deionised water will turn red with Schiff reagent. 3) For silver stain solutions, our lab

[Histonet] Murine Desmoplasia Markres

Dear Histonetters! I was wondering if anyone is working with Desmoplasia markers? I am working with xenograft models (human tumors implanted in mice). So the result is human tumor cells and Murine stroma. I am trying to test various desmoplasia markers found in literature, but almost all of them

[Histonet] re: water

I think that distilled water starts out at a ph of around 6-6.5, but usually DI water is closer to 7. Over time, both will drop in pH unless you buy specialized housing for the DI water. Since many people doing IHC at my university are using RO water, which has a pH similar to what the pH of the

[Histonet] re:water question

I think that distilled water starts out at a ph of around 6-6.5, but usually DI water is closer to 7. Over time, both will drop in pH unless you buy specialized housing for the DI water. Since many people doing IHC at my university are using RO water, which has a pH similar to what the pH of the

[Histonet] re:water question

I think that distilled water starts out at a ph of around 6-6.5, but usually DI water is closer to 7. Over time, both will drop in pH unless you buy specialized housing for the DI water. Since many people doing IHC at my university are using RO water, which has a pH similar to what the pH of the

[Histonet] wondering if anyone else has had this problem? wh...

wondering if anyone else has had this problem? when embedding small core bxs they are like they are charged and go off of the forceps to the edge of the embedding mold. its like they are running away from the forceps. what would be causing this? thanks so much. anita dudley providence

[Histonet] deplastisizing Spurr

I'm wondering if anyone has any helpful tips for deplastisizing Spurr. We currently use saturated Sodium Hydroxide in 100% alcohol. We tried a 5% and a 1% solution as well, but still not perfect. The main issue we are having is the tissue is lifting off of the slide during staining. For

[Histonet] Re: wondering if anyone else has had this problem?

Anita, I have seen this when using plastic embedding molds. The molds themselves hold an electric charge. It's annoying when your samples all migrate to the edges. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas

[Histonet] JCAHO - Universal Protocol

Dear Histonetters, Is there anyone out there that follows the JCAHO - Universal Protocol/Time Out procedure when the pathologist perform his/her own FNA's? We have a pathologist that will perform FNA's when asked and I was told that when he performs these procedures that we need to be following

[Histonet] Morgue lift

I'm hoping someone can help me. We are looking for a copy of the Operations Manual for a lift made by Lipshaw, Model 15BS. We use this for moving bodies in our morgue, and can not find information about servicing it. Thanks, Toni CONFIDENTIALITY NOTICE This message and any included

[Histonet] Calcium scaffold and decalcification

I have running a experiment in rabbit that included the bone reconstruction using mesenchymal stem cell seeded previously in calcium phosphate and calcium silicate scaffold. I need to know if, after some time, the scaffold remains in the tissue or they are reabsorbed and the bone is

[Histonet] RE: Quality Improvement

Laurie, I have a binder that is for Continuous Quality Improvement. If something comes up that is not part of my regular schedule of Quality Control or Quality Improvement it goes in that binder. I have a form that I use to state the reason for the Quality Improvement (what initiated the

[Histonet] microtome

What is the best microtome on the market these days? We are a research lab sectioning mainly mouse tissue, and mainly decalcified bone. We have beginners to experts in the lab all who will need to use it. It's been a while since I have been in the market for a microtome, so although I have

Re: [Histonet] microtome

We are also a research lab and do both decal and non-decal bone from mouse to horse.  We have three Leicas, 2 RM 2255 and one older RM2155.  They are all motorized and we love them with tungsten carbide or disposable knives.  We have them for 3 and 4 years with never a problem. Pam Marcum

RE: [Histonet] question regarding rules for disposal of slides cassettes

Thanks for the tip! I was sure thi= s list would have the answers. I actually didn't know about the archives un= til you mentioned it, and did a search for it just now. Thanks so much! Jenee S. Odani, D.V.M., Dip= l. ACVP Veterinary Medical Officer Hawaii State Veterinary =

RE: [Histonet] Quality Improvement

I am a firm believer in lab notebooks. In it we record all routine maintenance, quality control, solution preparation (especially dye lot numbers, source etc) as well as variations to technique caused by vagaries of dye reagents. Any developmental work is also recorded, trial of new dyes,

Re: [Histonet] Quality Improvement

Laurie, et al. - This is what we do in my lab... We use a computer quality control program called QPulse. Everytime an SOP is changed (even slightly, such as vendor or incubation time change), the new revision is made through the QPulse Document Draft program, and in the process of making

[Histonet] cassette storage/cardboard slide holder for sale

For Sale 2 set of paraffin block drawers $25 20 or better cardboard slide holders (holds 20 slides) $1 each Cathy Mayton Wasatch Histo Consultants, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] C4d Antibody

Dear People from Histonet Could you share your experience with C4d Antibody ? I couldn't get any results with the mouse monoclonal from Quidel. I will try now the rabbit polyclonal ( C4dpAb )from Biomedica - Grouppe. I work in a Public Hospital with many kidney transplantations