I will add to Damien's comments and say that another thing to consider
is the labeling schedule. Typically the smaller the animal the greater
interlabel time period. For example, a smaller window in a mouse tibia/
femur might make it difficult to determine the difference between
single and d
Hi Jamie,
Goal 1:
For fluorescent label measurements, you’re definitely better off measuring
multiple fields using a higher power objective (at least10X). Accuracy is
paramount when measuring inter-label width and working at a higher
magnification will help to ensure this. Your other option is to
hi ,
I wanted to know what would happen if i use Vector Blue and Vector VIP
chromogen substrate with Labvision's HRP and Alkaline phosphatase polymer
secondary antibody. Since these chromogens come as avidin biotin cojugated
enzymes which may cause reaction with endogenous Biotin.
Plz Advi
It was pretty funny alright. One for the Histonet Posts of Shame. There
are a few of us that could be runners-up with Ford. haha
Mark
On Fri, Mar 27, 2009 at 7:13 PM, Emily Sours wrote:
> I thought the rant was pretty funny.
>
> Just repeat to yourself "it's an email list, I should really ju
The problem has nothing to do with the organs being blood covered but with the
fact that they are entire and with little formalin. If they were sliced to
expose the interior and placed in enough formalin it is of no importance how
covered with blood externally they are.
René J.
--- On Fri, 3/27
Very good, Gudrun I agree with you.
I have a seen this crack also on the blocks after embedding them on the cooling
stage, and you separate the cassette from the mold. On the tissue and the
paraffin, you will see this type of cracking description that you mentioned.
In this case I will describ
I think we've gone over the terminology of alum hematoxylins more than
once on Histonet.
When you make up a working solution of what we call hematoxylin, you
dissolve the colorless dyestuff (dye precursor) hematoxylin in water.
With oxidation the colorless dyestuff becomes the dye, called hematein
If PIER is needed, we use Proteinase K, but we avoid it, if possible.
Citrate Buffer is historically the next buffer, that got generally used. Few
years later with investigations about Ca-Ions involved in fixation, the
EDTA-buffers with high pH appeared.
It was shown, that most of the diagnostic an
Does this look like desert-earth cracks? If yes, this could be a matter of
water under the edges after sectioning and half-drying. Do you find the
artefacts in the lower part of your sections related to the drying-direction
(upright)?
The section dries and sticks from the outer paraffin-limits to t
