At the moment I'm doing a large batch of myosin ATPase staining
on human skeletal muscle. 9.4 and the reversal at 4.3 are excellent but the
4.6 is not so good. I will alter the reversal pH in a range from 4.5 to 4.7
but I'm wondering, does the buffer have an effect. I use a glycine
The mandible and maxilla of chicken's' beaks are porous bone covered by horny
keratin so it is very likely that you will have to do a double procedure.
First to soften the covering keratin with 10% sodium or potasium hydroxide aq.
solution and later decalcify the bone.
Since it is a poroues bone
Hi Histonetters!
I hope you are doing well. I wanted to send a special bulletin wishing
you a Happy National Medical Laboratory Professionals Week. I hope your
lab has been having some fun, informative and special events to
celebrate because you deserve it.
I have heard of some really cool
Another thought about iron staining. It may be ferrous iron instead of ferric
in the beaks. Prussian blue staining uses potassium ferrocyanide, you might
also try using potassium ferricyanide for Turnbull's staining.
Paula
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The other approach of course would be to avoid decalcification
altogether and embed them in methacrylate, provided you are set up to embed and
section such blocks.
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All EM Techs. Which Ultra knife is better. Diatome or Pelco? Is there any
other company that I should consider. I plan to purchase an Ultra and a
Histo knife.
Thank You
Stella
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We always used Diatome and they also took care of resharpening our diamond
knifes. Always satisfied with their service.
René J.
--- On Thu, 4/23/09, Stella Mireles estellamire...@gmail.com wrote:
From: Stella Mireles estellamire...@gmail.com
Subject: [Histonet] EM Knife
To:
Sorry Histonetter's
But it is my Friday and wouldn't this method give new meaning to the term
sausage block. :) I wish Margaret all the luck in her quest and if it was me,
I would try either or both methods.
Roger Charles
Microbiologist
Pennsylvania Veterinary Laboratory
2305 N Cameron St
Roger
If I were you I would compress this into a block before fixation.
Can use paper up to wax but would not use paper covering in the embedded block
it will make cutting too difficult.
Alternative is to use sausage skin as the covering and process, embed and cut
with this.
Barry
-Original
What would be wrong with just submitting the ground sample as one would
with a curetting or similar - that is if the pieces are small, submit
them for processing in a lens paper packet or one of those paper histo
bags, then scrape the pieces off the paper and embed into paraffin as
usual? Perhaps
Hi Stella:
All of our knives are Diatome and I have no complaints. On the other
hand, I have been doing business with Pelco for many years with no
problems ever. It seems unlilely that they are making their own knives
so they are probably buying and rebranding. You will probably be fine
with
I bought a couple of Microms and so far they have been great. Wondering
if anyone has purchased a new rotary microtome recently for a decent
price that they like. I have bought a couple of used as well many years
ago from a guy I trusted no longer in the business. Any suggestions
there? I have
We are evaluating some of our grossing procedures and cut off times and I was
wondering what other people are doing.
Who does your grossing, pathologist, PA, or other qualified individuals?
Who does you accessioning, histotechs, support staff, or other?
What is your cut off time for grossing
Is anyone willing to share their protocol for processing fatty breast tissue?
It is difficult to find any documentation or processes that work really well.
Thank you in advance, you have all been extremely helpful to me!
Jennifer Bull
Histology Supervisor
jennifer.b...@nwpathology.com
Does anyone have a herpes control for which they'd be willing to trade? I
can trade just about any tumor or control material you might need
thanks
Mark Tarango
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I agree with Sally, why not just process as a curretting by loosely placing
pieces of ground meat in a cassette and embed in an appropriate sized mold
for your IHC needs. Paper will shred, it does not section well, if at
all.
Dolores Fischer
The information transmitted is intended only for
Can anyone recommend a brand or line of commercial control slides for oil red O?
Thanks,
Steve Joy, BSc. MLT
Research and Development Technologist
5B2.03 Anatomical Pathology
University of Alberta Hospital
8440-112 st
Edmonton Ab
T6G 2B7
Phone: (780) 407-8015
Fax: (780) 407-3009
Email:
Hi, we use
Rb a Hu Myeloperoxidase
Dakocytomat
A039829
It works great on rat tissue!
2009-04-24
TF
发件人： Smith Wanda
发送时间： 2009-04-23 03:16:36
收件人： histonet@lists.utsouthwestern.edu
抄送：
主题： [Histonet] Myeloperoxidase Antibody
Good Afternoon Everyone,
Where do you recommend
Steve:
I would think of keeping a frozen block of fatty tissue around and cutting
fresh control when you do the stain or throw some precut slides in the freezer
after fixation, as the specimen needs to be fresh frozen anyway and cannot come
in contact with any alcohols. Skin is an OK tissue,
I think producing a cytoblock with histogel or similar could help.
You can also take citratplasma and coagulate it to a small block with the
meat-pieces inside.
Gudrun
-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
I thing handling it like a curretting wrapped in lens paper or the
sausage ideas will both work well. I will add make sure your tissue is no more
than 3 mm thick when you process it. Probably better to be a bit thinner. I
imagine there will be some beef fat in there and assume it will need
this is hard to do because of the frozen section requirement. However, we
make smears out of mayonnaise when we have Oil Red O stain and it works
well. Good luck
JTT
- Original Message -
From: Steven Joy steven@capitalhealth.ca
To: histonet@lists.utsouthwestern.edu
Sent:
Frozen section of adrenal (from autopsy) is a great control. Freeze, cut
50-100 sections, put in a slide box, put in freezer, good for about a year.
Peggy A. Wenk, HTL(ASCP)SLS
Beaumont Hospital
Royal Oak, MI 48073
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
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