[Histonet] Question about DAB waste

We just purchased a Dako Autostainer and are in the process of figuring out how to get rid of the haz waste that we will be generating. The machine separates the haz from non-haz, thankfully. I've been communicating with our waste facility and have been having some difficulty. They want to know

[Histonet] RE: Question about DAB waste

We keep our DAB waste in a plastic container and give it to our waste disposal company every 6 months. No one has objected to this procedure. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Podiatric Medicine Miami Shores, FL -Original Message- From:

Re: [Histonet] Lean Consultants

Here's the website for consulting group we used: www.sprickstegall.com Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email

RE: [Histonet] RE: Question about DAB waste

We had ours analyzed by our Hazardous Waste Disposal Co. They provide labels for the drums. We have a drum for the waste from the special stains, the DAB waste, and the xylene waste from the recycler - labels all provided by the disposal company. j Joyce Weems Pathology Manager Saint Joseph's

Re: [Histonet] Job Openings

We have a part-time dayshift and a full-time night shift opening for a HT. If you're interested here's our web address. www.geisinger.org or call me directly. Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA

[Histonet] IHC - PGDFR reactive in Canines

Hello, Does anyone have experience using PGDFR in canines and if so, is there a canine species control that is known to react with PGDFR? Thank you, Sandy Sandra Cheasty Histology Supervisor UW-Madison School of Veterinary Medicine 608 263-1680

[Histonet] xylene substitues

just wanting to see what everyone's favorite xylene substitute is for clearing slides before coverslipping?? Gene Cleveland Clinic ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] xylene substitutes

We use Formula 83 ... we have had no problems in all phases. Gary -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of njoydo...@aol.com Sent: Wednesday, May 13, 2009 7:25 AM To:

Re: [Histonet] xylene substitues

N-butyl acetate aka butyl ethanoate. njoydo...@aol.com wrote: just wanting to see what everyone's favorite xylene substitute is for clearing slides before coverslipping?? Gene Cleveland Clinic ___ Histonet mailing list

RE: [Histonet] xylene substitutes

I second Gary's comments. We use Formula 83 in all phases also. Linda -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martin, Gary Sent: Wednesday, May 13, 2009 10:50 AM To: njoydo...@aol.com;

[Histonet] blood spots

Does anyone have protocols for doing histology and hematology on blood spots? Helen Johnson (he...@health.state.ny.us) IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law

Re: [Histonet] xylene substitues

We use Propar by Anatech. It works great. Lynette njoydo...@aol.com 05/13/09 10:25 AM just wanting to see what everyone's favorite xylene substitute is for clearing slides before coverslipping?? Gene Cleveland Clinic ___ Histonet mailing list

Re: Re: [Histonet] xylene substitues

tulane !!! 2009-05-13 TF 发件人： Lynette Pavelich 发送时间： 2009-05-13 23:49:59 收件人： njoydo...@aol.com; Histonet@lists.utsouthwestern.edu 抄送： 主题： Re: [Histonet] xylene substitues We use Propar by Anatech. It works great. Lynette njoydo...@aol.com 05/13/09 10:25 AM just wanting to see

[Histonet] 0.2M borate acid pH 7.0

My recipe for 0.2M borate acid is: Sodium Borate acid.10 H2O (molecular weight 381.42, Na4B4O4.10H2O) 19.07 gram H2O 1 Litre Adjust pH = 7.0 with 5M HCl Is this fine? 2009-05-11

[Histonet] peroxidase block

good morning, Is there anyone out there who has eliminated using Peroxidase Block on either IHC or ICC? Thanks, Gene Cleveland Clinic ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

Re: [Histonet] Question about DAB waste

We detoxify our DAB using this method from: HAZARDOUS MATERIALS IN THE HISTOPATHOLOGY LABORATORY, by Dapson Dapson, fourth edition, pg 184. 1. Prepare the following aqeous stock solutions: a. 0.2M potassium permanganate (3.16% or 31.6g KMnO4/liter) b. 2.0M sulfuric acid (11.2% or 112 mL

[Histonet] Question about DAB Waste

Have any of you tried the Dab/Out? It is a system that extracts DAB from liquid waste. The liquid passes through a cartridge and captures the DAB, then you can dispose cartridge as a dry solid waste and pour the rest of the liquid down the drain. It is known to be cost effective and

RE: [Histonet] Question about DAB waste

We were told that, in Miami-Dade County, we would have to apply for a license to do this. We gave up on the cumbersome licensing procedure. Allen A. Smith, Ph.D. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On

[Histonet] vacant post

Hello Histonetters My Immuno lead tech has left and I urgently need a replacement Its a full-time position and requires good IHC experience as well as routine Histo experience - an all-rounder Need someone with at least 7-10 years experience as its a Senior post Anyone interested? Send me your CVs

[Histonet] Looking for part-time Histology work in Phoenix AZ

Hi All, I am looking for part-time Histology work in Phoenix AZ. I am available for almost any shift, my current employer is very flexible with my hours. I have been in field for 17 years and know all aspects of Histology. Please respond to this email if you have any questions. Thanks!!!

[Histonet] mouse CD11c

Is anyone working on CD11c in murine tissues? If so, can we exchange emails? ... I have some questions. Andrea Hooper anh2...@med.cornell.edu -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] mouse CD115

Anyone working with CD115 in mouse? If so, may I contact you as I have a few questions? Andrea Hooper anh2006 @ med.cornell.edu -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] 2009 LSH Symposium/Convention-Additional Information

Fellow Histonetters, The Louisiana Society for Histotechnology is pleased to announce the 26th Annual Symposium/Convention: Your Histeaux Surplus Package June 12 13, 2009

[Histonet] Leica cryostat

Hello everyone, We have decided to purchase a refurbished Leica cryostat and are trying to decide which model to choose; does anyone have experience with the 1510, the CM 1800 or the 1800? Any feedback would be appreciated. Thanks! Janet ___

RE: [Histonet] Leica cryostat

We have an 1850 and like very much, but see if you can find one with the ultra violet light for sterilization. It would save a lot of hassle. Gary -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dertien,

Re: [Histonet] Toulidine blue stain

I have always liked a very simple method from an older edition of Humanson's book. 1. Bring paraffin sections to water. 2. Stain in 0.1% Toluidine Blue in distilled water, 2-5 minutes 3. Rinse thoroughly in distilled water. 4. Dehydrate in 2 changes of acetone, 5 minutes each. Dehydrating in

[Histonet] oxytocin receptor IHC fixation and storage

I'm sending this for a researcher here. He's got a lot of tissue in storage that's still 'wet' (still in various fixatives and bagged up). The tissues have been in fixative for over 10 years. He wants to know if he can still hang onto the tissue for future IHC use, or has it been in fixative