[Histonet] IHC cert.

Where can I find the study material needed to prepare for the IHC exam?  When on the ASCP website but found nothing.  ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] AFB Fites of cytospins

Carrie: The cytospin will not need dewaxing, as you point out, but I do not foresee any problem when compared with the control. You have to realize that dewaxing the control is intended only to expose the tissue section to the reagents. As to the peanut oil if you are trying to stain for common

[Histonet] Leica Peloris versus Microwave Processing

We are thinking about purchasing a new tissue processor. We have looked extensively into microwave tissue processors instead of the conventional tissue processors we are all used to. Recently two of my techs came back from a symposium and brought me literature on the Leica Peloris. The

RE: [Histonet] Leica Peloris versus Microwave Processing

Are there any issues with microwave processing and IHC? Thanks, Carol -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Tuesday, May 19, 2009 9:25 AM To:

[Histonet] RELIA Histology Jobs Update

Hi Histonetters! I hope everyone is having a great day. I wanted to do a quick post to tell you about some of the positions I am working on. All of my positions are full time permanent positions and my clients offer excellent compensation, benefits and relocation assistance. Here are the jobs I

RE: [Histonet] Leica Peloris versus Microwave Processing

We are using the Peloris and love it! I process small bx type specimens in 1 hour throughout the day. I process 80% of the rest of our specimens in 4 hours and the large fatty specimens run for 9 hours. What I also like is that I do not have to deal with any of the microwave safety regs and

[Histonet] Job Opening In Long Island, NY

** *Hello Histonetters,* ** *I have an opening for a Lead/Supervisory Histotechnologist in Long Island, NY. * ** *Please review the job description and send an updated resume with any other questions to aly...@alliedsearchpartners.com * *If you are not interested but you know someone who is, we

Re: [Histonet] Leica Peloris versus Microwave Processing

No issues using the Milestone products. Donna Sent via BlackBerry by ATT -Original Message- From: Carol Bryant cb...@lexclin.com Date: Tue, 19 May 2009 09:35:54 To: Vickroy, Jimvickroy@mhsil.com; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica Peloris versus

[Histonet] Autofluorescence

Hello Netters, We are trying to do do immunofluorescence on FFPE tissue sections; we always get autofluorescence. We have tried Sodium Borohydride treatment, does not work very well. Does anyone have good method to remove this autofluorescence from FFPE tissue sections. We have never tried

RE: [Histonet] Leica Peloris versus Microwave Processing

We use the Sakura Xpress with no problems. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Donna

[Histonet] ? potassium permangante filters, end point

Hello Netters, I was wondering if anyone has a reference or knows where I can track down information on determining the end point of potassium permanganate filters by dropping the used portion of the filter particulate into water. I have heard that if you drop the used filter particulate

[Histonet] Job opening in East Tennessee

Blount Memorial Hospital in Maryville, TN has a histology technician position open Monday thru Friday 7-3:30. We are located just minutes from the beautiful Smoky Mountains National Park and experience 4 wonderful seasons! All types of outdoor activities are possible. Maryville is host to the

[Histonet] RE: Autofluorescence with FFPE tissue

You wrote: We are trying to do do immunofluorescence on FFPE tissue sections; we always get autofluorescence. We have tried Sodium Borohydride treatment, does not work very well. Does anyone have good method to remove this autofluorescence from FFPE tissue sections. We have never tried Toluidine

[Histonet] HISTOLOGY, COVERSLIPPER; Microm CTM-6 Glass Coverslipper Cat. # 970010

Does anyone in histoland use this coverslipper? What do you like about it? What do you not like about it? Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email

[Histonet] Working with same host species primaries for double staining

We do sequential immunostaining to avoid cross reaction of a secondary antibody to the next primary antibody, and also perform a normal serum block that binds to the first secondary antibody: Two examples using two rat antimouse primaries and with immunofluorescent method. This is done on

[Histonet] MetAP-2

Has anyone successfully used Santa Cruz's MetAP-2 for IHC in FFPE mouse and rat sections? I am starting for scratch and am trying to find info on dilution range and antigen retrieval methods. The spec sheet only lists recommendations for applications other than IHC. Any info is greatly

[Histonet] Re Nikon Diaphot 300

Does anyone have a .pdf copy of the instructions manual for the Nikon Diaphot 300 Inverted Microscope they could send me? Thanks, Michael Norman The information contained in this email may be confidential and/or legally privileged. It has been sent for the sole

[Histonet] IHC certification

How do I go about finding the study material needed for the IHC exam, how do I prepare, etc. Thanks Ginny Achstetter ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Career Opportunity in Chicago area

Last week, my former employer, Loyola University Medical Center in Maywood, Il., layed off 440 employees (about 8 % of the workforce). I was the Manager of Anatomic Pathology (Histo/Cyto/Autopsy/Transcription/MohsLabs/Clerical Support) and now am looking for a career opportunity in the

[Histonet] Looking for career opportunity in Chicago area

I am looking for a career opportunity. - Forwarded Message - From: jimr0...@comcast.net To: Histonet2 histonet@lists.utsouthwestern.edu Sent: Tuesday, May 19, 2009 12:14:08 PM GMT -06:00 US/Canada Central Subject: Career Opportunity in Chicago area Last week, my former

[Histonet] Trying to post

just testing to see if I can post ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] CLIA regulations

Hello Histoneters, I was wondering if someone could contact me offline about CLIA regulations and/or inspections. I have a few concerns. Any help would be appreciated. Thanks in advance. JD jdhi...@yahoo.com ___ Histonet mailing list

[Histonet] PathNet HLA Module

I'm hoping to network with some wonderful Pathologists (and informaticists). I'm wondering if anyone out there has ever used or been a part of an implementation of Cerner Millennium's PathNet HLA module for tissue transplant. Any networking and advice is greatly appreciated!! Lori M. Brown,

[Histonet] PO/Zylene on cytospins for Fites

Hi Rene, Thanks for the info. We are doing a AFB Fites for Nocardia. There is filamentous bacteria in the patient GMS and the docs are differentiating the type. I did not deparaffinize the patient cytospin slides and I did deparaffinize the patient control with Z/PO. The problem I had been

[Histonet] Hi Tony,

Hi Tony, Thanks for the info. I understand the discussion topic. I just wish their was one straight answer! But then this is histology! Carrie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Thanks to those who helped find Larry M. plus

I was able to speak to Larry M. about my tissue processor problems. thank you all for your help. I am now looking for perhaps a broken MVP-1 or 2 since I am no longer able to get the CRT display parts to fix mine. they are interchangeable and if I could find a discarded MVP I

[Histonet] Cryoprotection Issues with Unfixed Mouse Hearts

Does anyone have any suggestions or protocols for cryoprotecting unfixed mouse heart tissue? Some of the ones I have been cryosectioning lately have been damaged, I think because of improper cryoprotection. Thanks! Adam baza0...@umn.edu ___

RE: [Histonet] Cryoprotection Issues with Unfixed Mouse Hearts

Adam, It is not a good to sucrose cryoprotect fresh tissues. Only well fixed tissues should be cryoprotected to lessen freezing artifact caused by large water ice crystals. Charles Scouten, on his website discusses this, and if one cryoprotects fresh tissues, you change the osmolarity within

RE: [Histonet] IHC cert.

For all information about certification exams (e.g., HT, HTL, SLS (specialist in laboratory safety), or on qualification exams (e.g., QIHC), go to www.ascp.org Click on Laboratory Professional Click on Certification Click on either Get Certified or Get Qualified Under Get Qualified, there is