Hi,
I would like to know if anyone has carried out MAP2 and GFAP double
staining? If so, I would appreciate if you could the antibodies used and the
detection method?
Thanks,
Anand Vasudevan
Nanyang Technological University (NTU)
Singapore
___
Histonet m
Just want to mention a point that some antigens are too fragile to PFA and in
my hand can not be retrieval"ed" anymore...such as rat CD31.
2009-06-08
TF
发件人： Patsy Ruegg
发送时间： 2009-06-08 00:13:46
收件人： 'Tony Henwood'; histonet@lists.utsouthwestern.edu
抄送：
主题： RE: [Histonet] Storage
Not sure how this got through histonet!!! This is not the first time something
like this has happened
- Forwarded Message
From: Jerry Meade
To: histo...@pathology.swmed.edu
Sent: Saturday, June 6, 2009 12:45:25 AM
Subject: [Histonet] Jerry sent you a private message on Tagged :)
Hatem:
Since this info hasn't already been offered, I'd suggest that you
considering using a 'programmable' water bath or pressure cooker -- where
the temperature is reduced to 65 to 75 degrees, but the exposure time
extended to three to six hours.
Sally
---
Date:
I didn't get very many response to this, so I will post again. Perhaps not
many have used this coverslipper? I am not sure of how well it sold when SP
distributed it?
Patsy
I am in the process of helping to set up a new histology lab and we want to
get a stand alone glass coverslipper. I kn
I agree with Tony, the problem is usually underfixation not over with
aldehyde fixatives, if you do not stabilize the proteins by fixing (Bryan
Hewlett says this takes 24 hrs. no matter the size of the tissue) paraffin
processing will adversely affect your tissue and in some cases the proteins
of i
I have done extensive IHC work on bone and cartilage samples and in my
experience, controlled enzyme digestion such as pepsin at 37dc flat on a
slide warmer, or trypsin, or proteinase K are my first choices for AR on
bone samples. Complete draining and airdrying is a must before heating the
slides
