[Histonet] aggrecan IHC on articular cartilage

Hi Everyone, I was wondering if anyone has done aggrecan IHC on articular cartilage from FFPE/decaled rodent knees.  The references and antibodies all call for a funky pretreatment step rather than a routine heat or enzyme (granted all of the references I have found are all for westerns and

[Histonet] used equipment

Greetings. We are in the market for a used gravity oven, probably about 2-3 cu. ft. in capacity. We would like to know what equipment remanufacturers you have used and what ones you liked. Thanks. Paul UroPartners, LLC ___ Histonet

[Histonet] FW: Cytokeratin 18 M30 apoptosis staining

Has anyone worked with this particular antibody? Can you give me any advise as far as what worked? I have an investigator that wants to use this antibody in diseased liver tissue and nothing that I am trying is working. Thanks! Laurie Laurie Popp, BA HT ASCP cm TACMA Shared

[Histonet] Cytokeratin 18 M30 apoptosis staining

Has anyone worked with this particular antibody? Can you give me any advise as far as what worked? I have an investigator that wants to use this antibody in diseased liver tissue and nothing that I am trying is working. Thanks! Laurie Laurie Popp, BA HT ASCP cm TACMA Shared Services Mayo

[Histonet] RNA In Situ Hybridizaton

My supervisor wants me to learn In Situ Hybridization using RNA probes in formalin fixed, paraffin embedded tissues. I have a Dako Autostainer, but I know that some of the procedure would be manual. I would appreciate any protocols that would help me. Thank you for your help. Pat Pat Bell

[Histonet] biocare bcl 6

Message: 3 Date: Mon, 15 Jun 2009 15:34:33 -0400 From: Justin Peters jpet...@bostwicklaboratories.com Subject: [Histonet] BCL-6 To: histonet@lists.utsouthwestern.edu Message-ID: 24d22de9e488aa43bf92a4389f2ddb1f05dfa...@mail1.bostwick.com Content-Type: text/plain; charset=us-ascii I

[Histonet] Synaptophysin

We use a IVD from Cellmarque and use pa ncreas for the control Benny Ba= binsack HT(ASCP) Lab Services Senior Tech,n= bsp;Pathology Department (740)454-5587 ***Confidentiality Notice*** The information contained in this e-mail message ([Histonet] Synaptophysin), including any

[Histonet] Glypican-3 on BondMax

Hi Histonetters! Has anyone been using Glypican-3 on the Leica BondMax platform? If so, please msg me, as I have some questions. I am having a tough time finding the right conditions... dilution, pretreatment, incubation times etc.. thanks Steven srwil...@gmail.com

[Histonet] Glypican-3 on BondMax

Hi Histonetters! Has anyone been using Glypican-3 on the Leica BondMax platform? If so, please msg me, as I have some questions. I am having a tough time finding the right conditions... dilution, pretreatment, incubation times etc.. thanks Steven srwil...@gmail.com

[Histonet] xylene and frozen sections

Lynn _ Insert movie times and more without leaving Hotmail®. http://windowslive.com/Tutorial/Hotmail/QuickAdd?ocid=TXT_TAGLM_WL_HM_Tutorial_QuickAdd_062009___ Histonet mailing list

[Histonet] Temp work

Hi everyone, I am looking for temp work. If you have availability on a travel basis let me know. I have 10+ years experience with histology and IHC in a research venue and am published! Cheers, Loralei ___ Histonet mailing list