RE: [Histonet] Sheep Lung

2009-06-25 Thread Kemlo Rogerson
Fixed or unfixed sheep lung? If fixed then you can perfuse with formalin from a header tank 1 metre above lung and then infiltrate with gelatine. Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace

RE: [Histonet] hood for manual special stains

2009-06-25 Thread Kemlo Rogerson
SKIN: Repeated contact can produce dermatitis (dryness and cracking) due to degreasing action. SKIN SENSITIZATION: Skin sensitization was not produced in any of 24 volunteers. There is one case report of a person developing an allergic skin reaction (contact urticaria) following exposure to

RE: [Histonet] Re: tracking turnaround time of intraoperativeconsultations

2009-06-25 Thread Garrison, Becky
Sorry I did not respond yesterday. The reason we just started measuring order to sign out for frozens was also prompted by preparation for our next JCAHO inspection. However, there is this distinction. The Joint Commission does not define a frozen as a critical test. The designation of

Re: [Histonet] Re: tracking turnaround time of intraoperative consultations

2009-06-25 Thread Andrea Grantham
Some years ago (before electronic ordering systems) when I worked in the clinical lab the specimen for frozen was time stamped when it came into the lab and the pathologist stamped it - or more likely the pathologist gave it to a histotech for this - when the result was communicated to the

[Histonet] Beta Amyloid

2009-06-25 Thread Drew Sally A
My co-worker previously asked about any versions of this antibody that didn't require formic acid pretreatment. I was wondering if there is experience out there using the antibody from DBS, and what the consensus is about the stability of the antibody (from any company) after being diluted. We

[Histonet] STD testing in Lab

2009-06-25 Thread Jill Cox
Hi Histonetters!!We are trying to bring in house STD testing in our lab and would like to know what type of equipment everyone uses and feedback. We would like to take on HPV, GC Chlamydia and gonorrhea. Any information would be greatly appreciated!! Thank you.. Jill Cox HT (ASCP)        

[Histonet] 100 mM vs. 300 mM phosphate buffer

2009-06-25 Thread Neil M. Fournier
Hello everyone, Is there a reason why people tend to use a phosphate buffer strength of 100 mM and not 300 mM when dissolving compounds for injections or preparing buffers for staining? Much appreciated Thanks Neil E-mail message checked by Spyware Doctor (6.0.1.441) Database

Re: [Histonet] STD testing in Lab

2009-06-25 Thread Greg Dobbin
Well I would check the local human rights laws. I'm not so sure your staff are going to be too thrilled with being tested for all these diseases! .I'M KIDDING of course. I'm sorry I can't contribute anything worthwhile (other than some comic relief) on what is otherwise a rather

RE: [Histonet] hood for manual special stains

2009-06-25 Thread Maria Katleba
Why would anyone open a new lab, give cytology a hood yet have a complete disregard for histology area? I am so glad I work for Queen of the Valley Medical Center... they actually do care about ALL of there employees, no matter what department they work in. To answer your question about fumes

RE: [Histonet] STD testing in Lab

2009-06-25 Thread Blazek, Linda
NO!! It's THURSDAY afternoon isn't it? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Greg Dobbin Sent: Thursday, June 25, 2009 2:20 PM To: Histonet@lists.utsouthwestern.edu Subject: Re:

RE: [Histonet] STD testing in Lab

2009-06-25 Thread Greg Dobbin
Oh my gosh! You're right. My day is suddenly not so drab!! LOL Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 I find that the harder I work,

[Histonet] re: Beta-amyloid

2009-06-25 Thread Hobbs, Carl
Have a look in the image gallery here : http://www.immunoportal.com/index.php I am interested to know why you want to avoid Formic acid AR. In my XP, Formic acid exclusively exposes plaque-beta amyloid, using appropriate Abs. However, whether or not I am right, Citric acid ( or whatever your

[Histonet] PRN work wanted in Madison, WI

2009-06-25 Thread Steven Coakley
I have a great LTE position as a Mohs tech at the UW .   Need a few more hours.  Anyone know of any labs needing an as needed HT in the Madison, WI area?   Thanks everyone,   Steve ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

RE: [Histonet] hood for manual special stains

2009-06-25 Thread Bernice Frederick
All, A good source for safety issues in the histo lab is Maureen Doran. She is the safety person for the NSH. I'm sure her contact info is on the site. If not I know it is on the Illinois site (www.ilhisto.org) She is always at the NSH symposium and usually does a class, not only on safety but on

[Histonet] Enzyme histochemistry for choline kinase

2009-06-25 Thread Connolly, Brett M
Hi all, Anyone know a way to stain tissues for choline kinase?? Thx, Brett Brett M. Connolly, Ph.D. Research Fellow, Imaging Dept. Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_conno...@merck.com Notice: This e-mail message, together with

[Histonet] re: control slide storage

2009-06-25 Thread Renko, Heather D.
Anyone have any published literature for reference on storage control; refrigerator vs. room temp vs. freezer. I have a friend asking for this and would also would love to get this information for my own reference I searched the archives but, came up with only one publication. Thank you in

[Histonet] Budget Microarray

2009-06-25 Thread O'Donnell, Bill
Good day Histonetters, A few weeks back, I posted that I had a method for making a tissue microarray on the cheap. I have received a lot of requests for how I do this. I wanted to write it up because, even though it is pretty easy to do, it is difficult to describe. Also, keep in mind that I

[Histonet] RE: re: control slide storage

2009-06-25 Thread Helen Fedor
Hello, We have a project that has worked on this topic using TMA's and have found that storing unstained, and unbaked slides at -20 enhances the preservation of antigenicity of the samples. It is not yet published but is getting written up. TMA slides that were stored for 5 years at -20 in very

[Histonet] Silver staining /other methods to reveal axonsprouting/retraction following injury

2009-06-25 Thread TF
Hi all i read such figures in Cajal's books..I guess he is not using specific degenerative sliver staining, as Natua developed the method 50 years later. I wonder anyone can send me the protocol for such kind of silver staining - there are so many ways of Golgi staining - to reveal post-injury