Re: Re: [Histonet] Re: IHC on frozens

2009-07-07 Thread TF
it is because when mentioning frozen section, we r talking about different procedures. some fix brain, sucrose sink, then perform OCT embedding and cryostat section;you can use enzyme digestion or HIER for antigen retrieval some other use fresh brain, snap freezing, and cryostat section; this

[Histonet] Tween 20

2009-07-07 Thread Merced M Leiker
Hi all, What is the difference between Enzyme Grade Tween 20 and Molecular Biology Grade Tween 20? Thanks! Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214

RE: [Histonet] Accu-edge sales rep

2009-07-07 Thread Bonner, Janet
I've had the same problem. I started keeping them in a ziplock with desiccant packets and it seems to help. Even then I have to bang them top-down to advance the blades to the top so they catch on to the slider mechanism. I don't know why I put up with this - except that I'm awfully

Re: [Histonet] Tween 20

2009-07-07 Thread Geoff McAuliffe
Ask the manufacturer, they will know. Or look at the MSDS Geoff Merced M Leiker wrote: Hi all, What is the difference between Enzyme Grade Tween 20 and Molecular Biology Grade Tween 20? Thanks! Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building

Re: [Histonet] Digital Slide Imaging

2009-07-07 Thread Geoff McAuliffe
We had our set of instructional slides for Histology (medical student course) scanned at 20X and are very happy with the results. Students load the slides and viewing program on their laptops and study wherever. Higher mag scanning (40X) is necessary for finer cellular detail. I don't know if

Re: [Histonet] Accu-edge sales rep

2009-07-07 Thread Geoff McAuliffe
I had a box of low profile blades with the same problem. The vendor sent me a new box. Geoff DiCarlo, Margaret wrote: Hello, I just opened a brand new box of Accu-Edge high profile blades and I cannot get the very first blade to slide out of the container. Even though I have had the box

[Histonet] RELIA Histology Management Job Alert 7/7/09 Histology Supervisor/Manager needed in San Antonio, TX

2009-07-07 Thread Pam Barker
Hi Histonetters!! I hope everyone had a safe and happy 4th of July weekend. I have a new position that I am very excited about and wanted to let you know about. This is a full time permanent position with one of my best clients. They offer excellent salary, benefits and relocation/sign on bonus.

[Histonet] Alk-1 controls

2009-07-07 Thread Webb, Dorothy L
Does anyone have a good source to purchase ALK-1 IHC controls from? We were getting them from Cell Marque, but their source is no longer available!! Thanks for any help!! Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 This e-mail and

[Histonet] Error corrections

2009-07-07 Thread kristen arvidson
How are people typically correcting errors that occur.  For example, mislabeled slides and path slips.  We do a lot of hand writing in our lab and when we make a mistake people are usually whiting out or scribbling.  I'm feeling like we shouldn't be doing thisis there a standard like a line

[Histonet] Hemo-d xylene substitute in processor

2009-07-07 Thread thecitan
Hi everyone. I was recently contracted to run a small routine derm lab. This is the first lab i've seen a lab use hemo-d as a xylene substitute. Are there any properties of this I should keep in mind while using it in processing / staining. Sent from my Verizon Wireless BlackBerry

[Histonet] CoPath Question

2009-07-07 Thread Richard Cartun
Periodically, we receive a call from an angry physician who wants to know why the specimen he or she sent to us has not been signed out. For those of you using CoPath as your Anatomic Pathology LIS, do you have a protocol (or system) for identifying cases that have not been signed out? We run

[Histonet] methylene blue microinjection

2009-07-07 Thread Salim Yalcin Inan
Dear All, Does anyone use intracerebroventricular methylene blue injection for the validation of drug/electrode injection/placement accuracy? Could you please give me the catalogue number of methylene blue, so I can order for myself? And any other advices would be greatly appreciated. Thank

[Histonet] ventana IHC and special stains users

2009-07-07 Thread Patsy Ruegg
Please for those of you using a Ventana IHC instrument and/or special stains instrument, I am interested in what you do to dispose of your toxic waste. I know each municipality is different so let me know what you do and where you live. Ventana does not separate toxic from non toxic waste so I