Re: RE: [Histonet] Bone marrows

Patsy Ruegg pru...@ihctech.net wrote: > I prefer formic acid decal for IHC work. You could make > your own 5% but I > always buy it from Decal Chemicals it is called Immunocal. Why? Does it cost you less to buy pre-diluted formic acid? John Kiernan Department of Anatomy & Cell Biology The Un

[Histonet] Cassette Blues

This list has been invaluable to me since returning part-time to histology after an 8 year hiatus. Thank you. Here's my latest dilemma. When I left the last job, I swear the holes in the plastic tissue cassettes were larger. I got to the point where I could embed and barely have to scrape any p

RE: [Histonet] DAKO Liquid DAB

I use Stable Dab from Open Biosystems, no mixing, you keep it in a freezer -20 but it does not freeze, it has methanol or glycerine or something in it that keeps it from freezing, you should not freeze DAB solutions. I love it and it is a lot cheaper than using Dako or anyone else. I also buy ant

RE: [Histonet] Histology Supplies

I would like to know about their charged slides as well, I told my techs to ask for samples to try before we buy but I do not think they have done that yet. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihc

RE: [Histonet] Bone marrows

Patti, I prefer formic acid decal for IHC work. You could make your own 5% but I always buy it from Decal Chemicals it is called Immunocal. I have found the rapid decal solutions are usually nitric acid and not good for IHC. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview

AW: [Histonet] Bone marrows

BM biopsies of 3mm diameter, Overnight fixation in NBF, 6-8 hours decal in a commercial mixture of formic acid and formaldehyd (about 5-10%, Richard Allen Scientific). Routine paraffin embedding. Good IHC results, Kappa-Lambda ISH possible. Gudrun Lang Histolab Linz, Austria -Ursprüngliche Na

[Histonet] Bone marrows

Hi All. I was wondering what everyone's current favorite bone marrow decal solution/method is. Including commercially available decal solutions. We will be doing IHC after fixation/decal. Thanks for the input (as always). Patti Loykasek BS, HTL, QIHC Clinical Lab Supervisor PhenoPath Laboratories

[Histonet] An SOP on Tissues, Slides and/or Wet Tissue?

Re-inventing the wheel is so passé - I need to write a pretty descriptive SOP on our policy of not releasing slides, blocks and (sometimes) wet tissue to other parties (keeping in mind we are a veterinary diagnostic and "reference" lab). The State policy for records (it does not specifically me

[Histonet] HSV Controls

Hi, Does anyone know of a source for HSV I/II positive paraffin control blocks? Thanks in advance. Tracey Lenek Temporary Supervisor - Anatomic Pathology Calgary Laboratory Services 403-770-3448 This message and any attached documents are only for the use of the

[Histonet] New CAP question on records and anatomic retention

Hello All: Just got our self inspection material and came across this new question dealing with ending lab operations. Has anyone, especially independent labs or hospitals not part of a big system, developed such a policy? Gen.20425 Does the laboratory have a policy to ensure that all rec

[Histonet] Histology Supplies

Hey everyone, I am looking at getting some supplies for a new histology lab. Has anyone ever purchased from Gorilla Scientific? www.GorillaScientific.com. I just got a flyer in the mail from them and it seems like they have some really good prices. I think I can save a lot on my slides if I purcha