[Histonet] immunohisto c-MYC and APC

Morning to All Please need some help. Anyone used the following antibodies on FFPE human tissue. Novocastra mouse monoclonal c-MYC(oncoprotein) clone 9E11 and APC (adenomatous polyposis Coli)clone EMM43. Have tried with and without retrieval. Tried different retrievals No staining. Thanks

RE: [Histonet] Lab Telephone

When our new lab was built they gave us phones that could only be used for dialing out...and none in the smaller, procedure rooms. We argued that the tissue processors and other instruments were often in these procedure rooms and it would be handy if we needed a service tech to walk us through a

[Histonet] Da Phone Issue

Thank you to everyone that replied to my (ridiculous) phone justification message! I have many very good, logical, sensible, common-sense reasons there should be a phone IN the lab and I will use every one of them in a short, bullet-point message to my boss. Another reason Histonet is such a

[Histonet] RE: Da Phone Issue

Is Ohio close enough? Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lbla...@digestivespecialists.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] Tech vs Professional

Does anyone out in histology have any documentation from CMS, ASCP, or NSH that breaks down the job duties or areas for what is covered under Tech and professional part of the CPT billing codes. For example is Grossing part of the a profee or tech component. Rick Garnhart HT(ASCP) Memorial

[Histonet] Evan's Blue - blood brain barrier

We will be infusing Evan's Blue to assess blood brain barrier disruption in some rats brains. The current plan is to follow this with saline perfusion to clear the vasculature and then perfuse with fixative. Brains will then be sunk in sucrose and frozen for sectioning, but I am wondering if

[Histonet] in situ with paraffin sections

Hello I've been reading a few protocols for RNA in situ hybridization with paraffin sections. One of the suggestions is to dehydrate the sections on the first day after rehydration and pre-hybridization processing (para fix, proteinase K, acetylation, pbs washes between all). This seems counter

[Histonet] job opening, Minneapolis VA

DEPT. OF VETERAN AFFAIRS MEDICAL CENTER, MINNEPOLIS, MN. Full time Histotech. opportunity at Minneapolis VA. BS or BA in Biology. HT cert. required, HTL preferred. Prefer 5 yr. exp. IHC experience a plus. Effective interpersonal skills required. Holiday, evenings and weekends off. Excellent

[Histonet] destaining bone

Hello All, I hope someone can assist us with a protocol for destaining some mouse ulna. A student stained his bones for 12 hours in 1% basic fuchsin in graded ethyl alcohol from 80 % , 90 % and then 100% as a prestain then into 100 % ethyl alcohol to destain some prior to pmma plastic

[Histonet] Amyloid staining on frozen muscle bx's

Hi, What stain works for amyloid on frozen muscle bx tissue? I did the a Congo Red stain without much success. Thanks Sharon sal...@hsc.mb.ca This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information.

RE: [Histonet] Please recommend xylene substitutes

I have used Clear Rite 3 for may years. It is not as forgiving as xylene when it comes to water, but if you keep it fresh you will be fine. Marianne Kitchell Novus INternational From: histonet-boun...@lists.utsouthwestern.edu on behalf of jennifer cresor

[Histonet] bone sections

Hi Everyone Could any one tell me how they keep bone sections adhered to slides when staining. I'Ve tried polysine slides, pva glue slides, different ways of drying the sections and for different lenghts of time but nothing seems to work for every section. I'm only staining them with HE.

[Histonet] anti-bromodeoxyuridine staining

I am having a problem with my anti-bromodeoxyuridine (BRDU) stain. I have done this for many years on chicken tissues fixed in Notox. Now I have rat tissues (Notox again) and sometimes I get everything staining (all nuclei) and sometimes I get next to nothing to stain. I have recently started

Re: [Histonet] bone sections

Are you working with resin or paraffin sections? Jack Sent from my iPhone On Aug 18, 2009, at 6:50 AM, Williams, Sean s.a.willi...@liverpool.ac.uk wrote: Hi Everyone Could any one tell me how they keep bone sections adhered to slides when staining. I'Ve tried polysine slides, pva glue

RE: [Histonet] in situ with paraffin sections

Emily, Unfortunately, there are a lot of superfluous steps involved in ISH. It reminds me of the initial IPX protocols. There were many unfathomable steps included that seemed to incite a feeling of magic required to obtain results. Simplifying the technique was one of the main reasons why

RE: [Histonet] Amyloid staining on frozen muscle bx's

Sharon, For successful amyloid staining with Congo red, formalin fixation might be required. I have not been able to find any references for this except that formalin is used in the demonstration of amyloid in fat aspirations (reference: Duston et al (1987) Am J Med 82:412-414) Have you tried

[Histonet] anti-GFP antibody

Hi all, I am looking for a good anti-GFP antibody. I plan on using it for dual immunofluorescence on mouse bone in paraformaldehyde fixed, paraffin embedded sections, most likely with some antigen retrieval. Here's the main problem: I want to use it with a goat-anti-mouse primary and in a