Hi all,
I'm looking into staining with two primary antibodies from the same host, in
this case goat. I've read a bit about this on Jackson Immunoresearch's
website, but I wanted to run by my idea to get an idea if this is at all
feasible.
I want to stain mouse tissue with antigen X and antigen Y.
Need help, looking for knife/blade holder for Leitz 1512 Microtome.
Does anybody know where to buy this, thanks
Bader
e-mail address: bade...@gmail.com
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One of our pathologists has inquiring whether there a reference lab that
offers the antibody HNF-1B?
Thanks for any thumbs-up!
Sally Ann Drew, MT (ASCP)
sd...@uwhealth.org
IHC/ISH Lab DB1-223, Mail Code 3224
600 Highland Ave.
Madison, WI 53792
Phone (608) 265-6596
Fax (608) 262-7174
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I find your images at
http://www.immunoportal.com/modules.php?name=gallery2&g2_view=keyalbum.KeywordAlbum&g2_keyword=P-ERK
Do you use same antibody to stain chick spinal cord, or is this another
one?
Santa Cruz sc-101761.
I tested it on FFPW sections of rat brain sections, after Citric acid HI
Dear Histonet-
We have used the monoclonal Cox-2 antibody from BD Biosciences (cat # 610204)
with success in FFPE tissues. We have used it to stain rabbit tissue, but it
works in human tissue as well. HIER with citrate buffer, and 1:200 dilution.
Jeanenne Ely, BS, HT(ASCP), QIHC
Chief Histolo
Could be worse...once upon a time a fire safety officer asked me to sign a
declaration that in the event of a fire i could get to an extinguisher easily.
I pointed out that this was not the case as I was at the end of a hall with no
extinguisher between me and the exit, so he offered the solutio
In a previous lab, we went through that. In order to keep the large nitrogen
tank in a small room that had 2 access doors, there had to be an oxygen
monitoring inside the access points. Oxygen level had to be at some minimal
level otherwise an alarm would sound. Just in case, with doors closed,
Our hospital does.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
rick.garnh...@memorialhealthsystem.com
Sent: Friday, September 04, 2009 13:07
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Pat
If your Pathologists bill for the professional component privately, and your
hospital bills for the technical component, who pay for expert
consultations of slides sent out?
Rick Garnhart HT(ASCP)
Memorial Health System
Histology Supervisor
1400 E. Boulder St.
Colorado Springs, CO 80909
Cell: 7
Happy Friday.
Does anyone out there do CD11b Staining on human tissue? If so which antibody
do you use?
Thank you,
Laurie
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We always do it that way, freeze 10ml & 50ml aliquots. We haven't
encountered any problems.
Iraz Toprak Aydin
EPFL SV ISREC
Lausanne, Switzerland
Quoting "Edwards, R.E." :
Is it OK to store freshly prepared 4% paraformaldehyde aliquots at -20C??
Thanks
I freeze my 4% PFA. Haven't had any troubles attributable to that yet.
Adam
On Fri, Sep 4, 2009 at 9:06 AM, Sherwood, Margaret
wrote:
> I routinely make up aliquots of Karnovsky's fixative (2.5%
> glutaraldehyde-2%
> paraformaldehyde solution) and freeze them. I have had no issue with
> fixatio
Check new OSHA regs and local regs. I have had some strange requests from
our inspectors over the last few years. They are probably looking at the
fact LN in a closed area with a leak could be fatal without realizing how
quickly it will dissipate. We had a driver years ago who lost a leg when an
Assuming that GMA blocks are stored under optimum conditions,i.e. with
desiccant and at -20C; how long can they be stored and still obtain meaningful
immunohistochemical staining?.
Many thanks for any info
Richa
Did they give a reason? Can they show you that requirement in writing
for Liquid N.? Not many places have outside ventilation.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard
Cartun
Sent: Friday, Sep
This is to prevent nitrogen suffocation in the event of a tank leak in a closed
space. Just more regs to make life harder. Put in an exhaust fan.
PKP
From: "Weems, Joyce"
To: Histonet
Sent: Friday, September 4, 2009 9:48:45 AM
Subject: RE: [Histonet] Storage
Not yet!! What in the world? J
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsou
We have been told by our Fire and Safety Department that we can no longer store
our large liquid nitrogen tank in a closed room (no windows or outside
ventilation). Has anyone else experienced this? Thank you.
Richard
Richard W. Cartun, Ph.D.
Director, Histology & Immunopathology
Director, Bi
I routinely make up aliquots of Karnovsky's fixative (2.5% glutaraldehyde-2%
paraformaldehyde solution) and freeze them. I have had no issue with fixation.
Peggy
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Beha
Is it OK to store freshly prepared 4% paraformaldehyde aliquots at -20C??
Thanks
Richard Edwards
Leicester UniversityU.K.
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Histonet
From: Sally Price
Subject: [Histonet] Endogenous biotin blocking
> Its been my understanding that blocking is only necessary when one is certain
> that background staining is caused by endogenous biotin, but maybe I'm
> off-base here. I look forward to eveyone's input.
I have been working on br
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