Hi all!
I'm hoping to get some help RE: purchasing a new ultra-low temp. upright
freezer. I've tried searching Histonet, but many of the posts are a few
years old, and my guess is that the products have changed a bit over the
years.
My lab is looking to purchase a large (absolute minimum size of
I worked with a pathologist many years ago who would use one piece of
polarising glass on the light source and wear his sunglasses. It worked fine.
regards
Tony
Tony Reilly
Chief Scientist
Anatomical Pathology
Pathology Queensland
Level 1, Building 15
Princess Alexandra Hospital
Ipswic
LOL that is good for a Thursday. But Friday's tomorrow. ;-)
--On Thursday, September 17, 2009 1:00 PM -0400 godsgal...@aol.com wrote:
Maria,
Thanks for the compliment...but unfortunately even I do not know
everything...
It was I that sent the original email.
Roxanne
-Origin
You can use glass camera filters. At the store they had a huge supply of
polarizing filters. I bought 2 for about $20 each that were the same brand,
but not even the same diameter. I put one on top of the slide and one on the
light source and it worked great. I also rotated the light source
One has to be careful in calling ALL cryoembedding mediums OCT since this is
a trademarked product from Sakura Finetek, under the name Tissue Tek OCT.
If you use other than OCT, then you need to give the name and specify WHICH
brand you are using in order to lessen confusion.
Out of curiosit
Maria,
Thanks for the compliment...but unfortunately even I do not know
everything...
It was I that sent the original email.
Roxanne
-Original Message-
From: Maria Katleba
To: godsgal...@aol.com ; histonet@lists.utsouthwestern.edu
Cc: Roxanne Fynboh
Sent: Thu, Sep 17, 2
Roxanne,
I got this question on the Histonet Blog... Since this is what you do for a
living, I was wondering if you could help us out here.
You can reply to ALL.
Maria Katleba
Queen of the Valley Medical Center
Napa CA
707-257-4076
-Original Message-
From: histonet-boun...@lists.utsout
Carol,
I have heard of similar problems. I believe the temperature of your cryostat
may be one of the reasons for your problem along with the type of media.
Optimal cryostat temps for cutting unfixed tissue such as brain, lymph node,
liver and kidney should be -12- 16 degrees. Fatty tissue such a
Carol,
I use OCT from a company US Laboratory Supplies It seem to do the job, no
residue, light refractive. I have tried to switch to lower cost OCT, such as
Tissue Tek, but I think some companies have been cutting corners in
manufacturing.
Sue
--
Message: 2
Dat
Hi Histonetters!
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in need of a Research Investigator. They are looking for someone with a
PhD,
I haven't been receiving any emails???
April Sachau, Account Manager
Laboratory Division
C&A Plaza= , 13609 California Street, Suite 200
Omaha= , NE 68154-5233
(402) 891-1118 x6047
(800) 856-0741
asac...@aureusmedical.com
www.aur= eusmedical.com
Ref#[
That seems to be a protocol for a very specific situation but incubation over
ice is absolutely not necessary and if done like that the incubation time has
to be prolonged substantially.
An incubation tie of 5 min at room temperature (as in the DAKO autostainer) is
all that is required.
René J.
Dilute 6 mg of DAB in 15 mL of PBS buffer pH7 and add 200 µL of 3% hydrogen
peroxide just before using and incubate at room temp.
René J.
--- On Thu, 9/17/09, Nancy Herman wrote:
From: Nancy Herman
Subject: Re: [Histonet] DAB Chromogen
To: "Nancy Herman" ,
histonet@lists.utsouthwestern.edu
D
Hi everybody!
A friend recommended this forum to me as he said lots of experts here could help
me with my horrible cryosections.
Right now, I am working on a gene expression pattern in zebrafish retina. I need
to section the eye before I see the expression clearly. So I do the whole mount
in sit
And Higgins brand is the best to survive the solvents. j
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Thursday, September 17, 2009 11:04
To: histonet@lists.utsouthwestern.edu; Dr. Fra
Be sure you get waterproof india ink as they now sell two kinds. Pam Marcum
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Thursday, September 17, 2009 11:04 AM
To: histonet@lists.utsout
They are talking about the common Indian ink, nothing else and you will find
it, as you write, in a office supplies store.
René J.
--- On Thu, 9/17/09, Dr. Frauke Neff wrote:
From: Dr. Frauke Neff
Subject: [Histonet] indian ink
To: histonet@lists.utsouthwestern.edu
Date: Thursday, September 1
Frauke
In the United Kingdom Cellpath and Thermo Shandon both sell tissue marking dyes
in a range of differing colours we have used both versions with no problems for
marking resection margins prior to processing.
Hope this helps
Peter
Peter L Craven FIBMS
Pathology Department
Raigmore Hospital
Dear everyone,
when I was working in pathology gross section lab, we used black dye
(and blue, green etc,"Tusche" in german) for orientation of the gross
section specimens. We fixed the dye with Bouin's solution. Now I was
asked for this dye, but I don't know if this was the "conventional"
Sorry, forgot to mention we had no staining results for the Tris-DAB protocol.
>>> "Nancy Herman" 2009/09/17 8:12 am >>>
I have this DAB staining protocol for IHC. It was used on the DAKO autostainer
and incubated for 5min. Our controls using the DAKO DAB chromogen kit worked
fine. Does anyo
I have this DAB staining protocol for IHC. It was used on the DAKO autostainer
and incubated for 5min. Our controls using the DAKO DAB chromogen kit worked
fine. Does anyone have any experience with a DAB protocol similar to this or
have any other information to add to this protocol? Is incu
HI HISTONETS...IM USUALLY SENDING FEW OF MY SAMPLES FOR IHC OUTSIDE THE
LAB...IS THERE SOMEONE THERE INTERESTED TO ACCEPT AN ENDOMETRIAL BIOPSY FOR NK
CELL STUDY???PATIENT IS VERY SPECIFIC EVEN SIMILAR TEST ALREADY DONE LIKE
CD8,CD20,CD3,CD16,CD56ANYBODY??
__
Correctthe formalin is neglegable.? We have been using this antobody for
years with minor issues.? Over the past few weeks we are running into a problem
with false negatives.? The control is working fine.
-Original Message-
From: Greg Dobbin
To: godsgal...@aol.com; histonet@lists.u
That's how often you have to turnover the water in a Koi Pond; 4 times and
hour. Do you keep fish?
Kemlo Rogerson
e-mail kemloroger...@nhs.net if not at work.
DD 01934 647057 or extension 3311 Mob 07749 754194;
Embrace uncertainty. Hard problems rarely have e
"Alcohol based" suggests to me an absence of formalin. In which case,
epitope retreival is not indicated.
Also, you didn't say if it had been working and stopped or if this was
a new marker for your lab.
Greg
Greg Dobbin, R.T.
Chief Technologist, Anatomic Pathology
Dept. of Laboratory Medicine,
Qu
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