Alcohol based suggests to me an absence of formalin. In which case,
epitope retreival is not indicated.
Also, you didn't say if it had been working and stopped or if this was
a new marker for your lab.
Greg
Greg Dobbin, R.T.
Chief Technologist, Anatomic Pathology
Dept. of Laboratory Medicine,
That's how often you have to turnover the water in a Koi Pond; 4 times and
hour. Do you keep fish?
Kemlo Rogerson
e-mail kemloroger...@nhs.net if not at work.
DD 01934 647057 or extension 3311 Mob 07749 754194;
Embrace uncertainty. Hard problems rarely have
Correctthe formalin is neglegable.? We have been using this antobody for
years with minor issues.? Over the past few weeks we are running into a problem
with false negatives.? The control is working fine.
-Original Message-
From: Greg Dobbin gvdob...@ihis.org
To:
HI HISTONETS...IM USUALLY SENDING FEW OF MY SAMPLES FOR IHC OUTSIDE THE
LAB...IS THERE SOMEONE THERE INTERESTED TO ACCEPT AN ENDOMETRIAL BIOPSY FOR NK
CELL STUDY???PATIENT IS VERY SPECIFIC EVEN SIMILAR TEST ALREADY DONE LIKE
CD8,CD20,CD3,CD16,CD56ANYBODY??
I have this DAB staining protocol for IHC. It was used on the DAKO autostainer
and incubated for 5min. Our controls using the DAKO DAB chromogen kit worked
fine. Does anyone have any experience with a DAB protocol similar to this or
have any other information to add to this protocol? Is
Dear everyone,
when I was working in pathology gross section lab, we used black dye
(and blue, green etc,Tusche in german) for orientation of the gross
section specimens. We fixed the dye with Bouin's solution. Now I was
asked for this dye, but I don't know if this was the conventional
That seems to be a protocol for a very specific situation but incubation over
ice is absolutely not necessary and if done like that the incubation time has
to be prolonged substantially.
An incubation tie of 5 min at room temperature (as in the DAKO autostainer) is
all that is required.
René J.
I worked with a pathologist many years ago who would use one piece of
polarising glass on the light source and wear his sunglasses. It worked fine.
regards
Tony
Tony Reilly
Chief Scientist
Anatomical Pathology
Pathology Queensland
Level 1, Building 15
Princess Alexandra Hospital
Hi all!
I'm hoping to get some help RE: purchasing a new ultra-low temp. upright
freezer. I've tried searching Histonet, but many of the posts are a few
years old, and my guess is that the products have changed a bit over the
years.
My lab is looking to purchase a large (absolute minimum size of
