Good Morning Histonetters-
First question: Textbook says tissue should remain in paraffin the shortest
time necessary for good infiltration because exposure to prolonged heat causes
shrinkage and hardening. Can anyone define exposure to prolonged heat? Is
that an hour? Three hours? Sitting
Hello,
We are considering the Leica Bond and their reagent rental or acquisition
option, and I have a question regarding back orders.
If anyone places an order through Leica for their Bond reagents (novocastra),
are there any problems with back orders on antibodies, detection system and
We get almost all their antibodies and ancillaries and have never had a
problem! They are a great company and we love the Bond Max.
Josie Britton HT
Cheshire Medical Center
Keene, NH 03431
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
We have never had a backorder problem- have 2 Bondmax's over 2 years. I love
them.
Jo Mauger
Josie Britton jcbrit...@cheshire-med.com 10/05/09 11:29 AM
We get almost all their antibodies and ancillaries and have never had a
problem! They are a great company and we love the Bond Max.
We've had no problems with either Bond or Novacastra orders.
Joyce
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula
Lucas
Sent: Monday, October 05, 2009 11:20
To: histo...@pathology.swmed.edu
Subject:
Almost always receive on time. I can think of only one instance in a
year and a half where anything was on back order (it was an antibody).
Greg
Greg Dobbin, R.T.
Chief Technologist, Anatomic Pathology
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PEC1A
After you have developed a processing protocol and obtained good infiltration
after a certain time (hours) in paraffin, any and all the time above that
period of adequate infiltration = exposure to prolonged heat.
Some histotechs even don't fill the holding chamber in the embedding center, a
Nancy,
Tissue should be processed @ between 60-62 degrees centigrade. We have
three paraffin baths. The 1st bath is set for 45 mins, the 2cd and 3rd
are for 1 hour each. This is for large specimens. Small specimens are
for 30 mins. the first two baths and 45 mins for the last. It is very
There is nothing you can do to make brain frozen sections acceptable. Your
Pathologists need to learn how to read smears, or just accept being wrong 50%
of the time. An educated guess based on the imaging is more accurate than
frozen sections on intra-axial primary brain tumors.
Richard B
Dear Histonetters,
I hope you can help me. I'm working with mouse embryos. Now I have some
samples (~ E.10.5), which have already gone through ISH. I tried to
make some paraffin sectionings from them, but most of the sectionings were
compressed anddid't look very nice. I tried the
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