[Histonet] CD 31 in rats

Hi Patsy I have tried RECA-1 and not had great success. The Fitzgerald one is the best and we have researched this plenty. One option for PFA fixed rat tissue is the Abcam VWF ( ab6994). I use it 1:400 overnight incubation of primary with 10 minutes proteinase K before hand, followed by

[Histonet] Leica Stainer Question

I use the Leica AutoStainer XL (and love it). I need to program it for a run FROM water through the HE part through to XYL. I have a program TO water for special stains, IHC, etc., but have not programmed it for a run when I mess up and forget to reset for the routine HE run after I've done a

[Histonet] Beckstead ZSF FW: Zn fixative 550523 vs 552658

Follow up to the Beckstead Zinc IHC fixative saga.BD Pharmingen states that their IHC Zinc fix does contain the proper ratio/ingredients to make it identical to the Beckstead fix. See below...Thanks to everyone!! Kathy Cormier Histology Manager Charles River Laboratories 251 Ballardvale

[Histonet] Paraplast

Has anyone noticed changes in tissue fluorescence if using Paraplast, Paraplast plus or Paraplast extra? E.g. increased autofluorescence, increased or decreased signal after staining, anything really. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson

[Histonet] PRN.POSITION

Hello, We are seeking a PRN histotech for Tuesday and Thursday of each week,6:00 am to 2:30 pm. Must be a team player and be flexible to fill in other shifts when needed,other shifts would be 7:00 am to 3:30 And 8:30 am to 5:00pm Monday thru Friday only. (when other employees need time off)

[Histonet] Uranyl nitrate

Our Safety people are asking us to stop using uranyl nitrate which I believe is used in our Dieterle and Reticulin histochemical stains. Are there alternative staining protocols that do not use uranyl nitrate (forgive me because this is not my area of expertise)? Thank you. Richard Richard

RE: [Histonet] RE: Antigen retrieval question

Thanks to all who replied to my question. It is not our usual procedure to leave slides overnight in buffer after AR and not one that we will ever repeat. Brett Brett M. Connolly, Ph.D. Research Fellow, Imaging Dept. Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501

[Histonet] RE: Antigen retrieval question

Brett, how long? I've left them overnight before proceeding without any problems. It would not be re-fixation as with formalin. However, if it is in a detergent buffer it might cause some other kind of denaturation. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco,

[Histonet] Re: looking for CD31 for rat tissues

Hi Patsy Yes it works very well. The antibody is cat # RDI-RTCD31-3A12 from Fitzgerald. Yes it is zinc fixed. You use alk phos instead of DAB. See pic. Zinc Fixative (JB Fixative or ZSF) 0.1M Tris Buffer, pH 7.4 Tris Base 12.1 g (TRIZMA) 1N HCL

[Histonet] llEICA sTAINER

You can just start step one from water as your first step. Step one Station wash1 for example. We use that for frozen sections Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Mgr, OMW, Area 4, Lab 2438 301.398.4745(vm) 301.398.6360(lab) 301.398.9745(fax) -Original Message- From:

[Histonet] IHC protocols for GPI-linked proteins

Hi All, I've been searching for special IHC protocols for GPI-linked proteins to try to hold specific staining at the plasma membrane, but I haven't found anything useful. Would you have any suggestion that might be able to solve this problem? It would be very nice to here from you. Thanks,

[Histonet] Leica Auto Casette Writer

Dear Histonetters, Has anyone experienced any issues (adverse) with the Leica casette writer? Any issues with scanning the barcodes it produces? Any issues with the ink spilling? Thank you all! Chris Simmons, B.S., A.S., HTL(ASCP) Lead Technologist PUH Histology 412-647-7660 desk 13242 pager

[Histonet] Clear Paraffin? (Monfils, Paul)

IMEB, INC. had a clear paraffin at the NSH meeting in birmingham. They are at www.IMEBINC.com E-van From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu

[Histonet] Wet block transport containers?

Hi, We’re looking for rectangular containers to transport Sakura tissue processor cassette baskets filled with wet-tissue cassettes. The container has to be water-tight as it will have formalin in it. Is anyone using a container that fits that criteria? Everything we’ve tried so far leaks

[Histonet] Wet cassette transport containers?

Hi, We're looking for rectangular containers to transport Sakura tissue processor cassette baskets filled with wet-tissue cassettes. The container has to be water-tight as it will have formalin in it. Is anyone using a container that fits those criteria? Everything we've tried so far leaks

RE: [Histonet] Wet cassette transport containers?

Tim, We have also been using the locking plastic containers that have a rubber gasket around the lid. We place the Sakura rack inside of this container and then place it in a larger container of the same design and this is placed in a third container or an ice chest with formalin neutralizing

[Histonet] Trichrome Stains on MMA thin sections

I am having problems with both the Goldners and Massons Trichrome on my MMA sections. They are ground to between 20 and 30 microns, but the staining is nonspecific. It stains some of the bone the correct color, but not the rest of it. Any and all help would be greatly appreciated. Thanks,

[Histonet] Leica Imaging Workstation Q550MW

If anyone has a specification sheet (not brochure) for the Leica Q550MW Imaging system, would you contact me directly?   The information I'm looking for is dimensions (W/D/H), weight , electrical information, etc. All I've found is the brochure which gives none of the information that I

RE: [Histonet] Leica Auto Casette Writer

We are using that printer and works well for us. Regards, Marbella Chavarria BS, HTL (ASCP) Laboratory Supervisor CorePlus, LLC. 9450 S.W. 72nd Street Miami, FL 33173 Main:305-265-8300 ext 128 Fax: 786-924-0277 E-Mail: mchavar...@coreplus.com visit us online at:

[Histonet] Re: Fite control slides

The Fite (or Fite-Faraco) stain is a light-microscopic acid-fast stain with a different acid-fast extraction routine. (I don't know if it's ever been adapted for fluorescence microscopy, but it certainly could be.) The Fite stain is used to demonstrate Mycobacterium leprae (the etiologic agent of

[Histonet] Anyone CLIA Certified?

We are a derm lab who is CLIA certified.  We are going through some admin changes and I have been reading through (and learning) clia, osha, chemical hygiene etc  manuals and noticing they all seem outdated.  I am a lab sup that is responsible for all this and not quite sure how to go about it

[Histonet] Warthin Faulkner method for spirochaetes

We are trying to work up this stain per pathologists special request. We usually do the Warthin-Starry method not this modification. I have the published article from Stain Technology Vol. 20 No. 3 July 1945. The protocol is a little vague and I have some questions? Step one brings the

[Histonet] Stainer programming thanks

Thanks to several of you that responded to my how-to-program-my-stainer question. I had a call from Carolyn Doan of Leica, who walked me through the (very simple - wouldn't you know?) programming and I was up and running in about 30 seconds. Thank you to all who advised me on copying and using

Re: [Histonet] Uranyl nitrate

Under separate cover I am sending what you are looking for. René J. --- On Wed, 10/21/09, Richard Cartun rcar...@harthosp.org wrote: From: Richard Cartun rcar...@harthosp.org Subject: [Histonet] Uranyl nitrate To: Histonet histonet@lists.utsouthwestern.edu Date: Wednesday, October 21, 2009,

[Histonet] Floor Cleaning

Hi Folks, Got a floor cleaning question for you. We are transitioning to a new floor cleaning crew in our histo lab. I've been asked by the building manager to elicit some opinions about products to use and/or techniques to best get paraffin up off of linoleum. We were fine with our previous

RE: [Histonet] Re: Fite control slides

NSH may have some in their control bank. Newcomer Supply sells control slides. Sigma may also. BErnice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From:

[Histonet] RE: Antigen retrieval question

If we need to hold retrieved slides for awhile we hold them in water up to 4 hours. Holding in water overnight leads to no signal. We have also tried holding the slides in our Tris buffer and have found decreased or no staining. Margaret Perry ___

[Histonet] withdrawal

Hi Please help me to withdrawal from the histonet. I am frustrated to see a huge amount email each day. Thanks yali ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] one day call for resumes Gaithersburg MD

For anyone interested in a Pathology Associate position at Medimmune in Gaithersburg Md, I am looking at resumes on Thursday of this week and will call folks Friday if interested. I just want to see if there is that special someone out there that I have missed over the past few months. The job

RE: [Histonet] withdrawal

To subscribe or unsubscribe to the list or change your subscription to the daily digest mode etc. you need to go to: http://lists.utsouthwestern.edu/mailman/listinfo/histonet or email: histonet-reque...@lists.utsouthwestern.edu with the word help (without quotation marks) in the subject line

[Histonet] Inspection question

Hi All. Happy Wednesday. Has anyone everyone had an auditor/inspector note that plants in the histology laboratory are a possible contamination hazard must be removed? Just wondering. Patti Loykasek BS, HTL, QIHC Clinical Lab Supervisor PhenoPath Laboratories Seattle, WA This e-mail

RE: [Histonet] Inspection question

No... And spider plants and some others help remove formalin fumes. That was published somewhere. j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From:

[Histonet] Digest replies

Wow, I'm tellin ya the next person that replies to a digest without trimming...after the day I've had I can't be responsible for the ball of flame that may be hurled! GRRR, Amos -- Sent from my mobile device ___ Histonet mailing list

RE: [Histonet] Inspection question

Not to mention a little organic bit of the outside world when your stuck inside a white-washed cube all day... Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Thomas Jasper Sent: Wed 10/21/2009 5:29 PM To: Weems, Joyce Cc:

Re: [Histonet] RE: Antigen retrieval question

hold in PBS as long as several days without any problem, under 4 C. 2009-10-22 TF 发件人： Perry, Margaret 发送时间： 2009-10-22 04:14:31 收件人： histonet@lists.utsouthwestern.edu 抄送： 主题： [Histonet] RE: Antigen retrieval question If we need to hold retrieved slides for awhile we hold them in

[Histonet] RE: Granzyme b and F4/80

For Serotec F4/80, we use 10% rabbit serum as block and 5% of the same as antibody diluent -our secondary is a biotinylated rabbit anti rat. We also retrieve with Dako proteinase K rather than citrate, with simple HRP-streptavidin then DAB, and it all works very nicely, no background issues.

RE: [Histonet] Wet cassette transport containers?

We transport wet cassettes to the core lab and had used plastic containers, but the plastic always hardens, cracks and the cost to continually replace was prohibitive. We have had great success using the Kpak bag sealing system and use a heat sealer to seal the bag. The sealed bag works well