Try extending the time in Bouins. Tendon is rather dense, and may need
longer time in the post-mordant. 60 degrees C for 1 hour, with a good 5-10
minute wash in running water afterwards, to remove all the yellow.
The same with the phosphotungstic acid rinse between the red and blue dyes.
Make it 5
My thanks to everyone for their input about cleaning lab floors. Much
appreciated.
Tom J.
Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology Services
Bend, Oregon 97701
541/693-2677
tjas...@copc.net
___
Histonet mailin
Jennifer,
Your NS background could be coming from endogenous biotin. I use the rat
anti ms F4/80 on ffpe mouse tissue all the time and it is very clean but I
use a labeled polymer detection system rather than AB. I use rat on mouse
hrp or ap labeled polymer detection from BioCare, or I use my ow
Sheila Hass at Micro Path Laboratories [where?} asks: >>Could someone
recommend a xylene substitute and mounting media to use for staining
and coverslipping (no vendors please)? I am considering processing
without xylene, and would like to discontinue it everywhere if
possible.<<
We've discussed t
It's really important to clean up as you go, when you gross tissues.
An overworked Pathologist's Assistant (PA) - who never sees the slides
- can carry over an awful lot of tissue and not realize the problem
that creates.
By ancient custom one may only have one of each kind of tool -
scalpel, scis
Jennifer,
I don't think, that you can substitute Biebrich Scarlett with Eosin. These
dyes have a different chemistry. That is well described on Bryan Llewellyn's
website: http://stainsfile.info/StainsFile/stain/trichindex.htm
The staining of collagen is also a matter of differentiation of the dyes
