Histonetter's in the VA, MD and DC area...reminder of the fall
mini-LEARN and EARN to be held this Saturday, November 7th 2009, at the
Virginia Beach Higher Education Center. Six (6) CEU's available, Two
Speakers: Carol Barone HT(ASCP) BA - Frozen Sections: Materials ,
Methods and Mastery (3 CEU's)
Miriam,
do you do FISH on bone marrow trephine biopsies, that have to be decalcified
before cutting and staining?
If decalcifing is performed with acid like formic acid or even hydrochloric
acid, DNA is degraded to smaller fragments. Successfull hybridization of
this fragments is kind of chance, b
Helo all,
We do FISH assay on FFPE tissue with "spot light tissue pretreatment" of
invitrogen. It do works on most of the tissues, but not on Bone Marrow tissue.
Do you have any idea?
Thanks
Dr. Miriam Birnbaum
Pathology
Asaf Hrofeh Medical Center
Israel
