hi...that one works...according to my collegue
BUT...dont use PFA fixation
u can do frozen section, or parrafin section with Zinc fixation
2009-11-13
TF
发件人： Nicholas David Evans
发送时间： 2009-11-13 09:24:09
收件人： histonet@lists.utsouthwestern.edu
抄送：
主题： [Histonet] CD31/PECAM antibody
Hello,
Can anyone suggest a good antibody to recognize mouse CD31 (PECAM)? I have
been trying to use BD Pharmingen MEC 13.3 with little success. I use
vector lab's goat anti-rat (BA-9400) but get loads of background on mouse
sections with this secondary. I also tried a mouse-on-mouse kit and sa
I had the great privilege to speak with Russ Allison at a conference in
Australia several years ago. He was a funny man, he was a wild man and
enjoyable to be around. His birthday was October 31st - and a Halloween has
never gone by since or will go by that I won't think of him. Cheers Russ!!
Hi Vinnie and anyone else who is in my address book,
I am so sorry! This has become a nightmare. I have tried to contact everyone,
but I must have at least close to 300 people in my address book.
Akemi
--- On Thu, 11/12/09, Della Speranza, Vinnie wrote:
From: Della Speranza, Vinnie
Subject:
I'm sorry to hear that. I met Russ several times at NSH over the years and had
several email correspondences with him. He was always helpful and a great guy.
Tim Morken
Supervisor, Histology / IPOX
UCSF Medical Center
San Francisco, CA
-Original Message-
From: histonet-boun...@lists.u
I also received the scam message purportedly from Akemi so something is amiss.
I can only assume Akemi that your address book/contact list has been acquired
by whoever is behind this.
Vinnie Della Speranza
Manager for Anatomic Pathology Services
165 Ashley Avenue Suite 309
Charleston, SC 29425
t
Vintage histonetters will remember the valuable contributions to our body of
knowledge made by Russ Allison. I was saddened to hear of his recent
passing after a seven year battle with Alzheimers. I'll not presume to
eulogize him, there are many who knew him better and longer, suffice it to
sa
I think that the tissues have been too long in the alcohol. I would suggest
hydrate the tissues and process them again with less time in the alcohols.
René J.
--- On Thu, 11/12/09, John Shelley wrote:
From: John Shelley
Subject: [Histonet] Tissue falling off slide
To: "histonet@lists.utsouth
Hi John,
In the past for IHC I used Surgipath StaOn in the waterbath for tissues that I
thought may wash, usually the bloody ones. I do not do IHC anymore and found
that the positive charged slides I had left over did not work on the autopsy
blocks I now cut for the ME. Sta-On works better t
Hi Histonetters,
This is a second request for help. I know that this is a pretty rudimentary
question but I need help. I have is group that has given me 175 mouse heart
specimens for processing and embedding and returned the specimens for them to
start microtoming them and the tissue is fallin
Aren't you the one who also sent the whole of Histonet an untrue urban-legend
email forward a while back?
You might want to consider being a tad more careful with your online doings.
Especially anything that could tie up an entire mailing list with your
indiscretions...
- Original Message
Thank you all for your responses. I have tried a few things. But still going at
it. :-) Have a great day!
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
New lab opening in Olympia, Washington needs a fulltime HT or HTL. GI practice
offers benefits, very flexible hours, and ideal working conditions. Must have 3
to 5 years experience. Please contact me if you are interested.
Konni Black
Pathology/ Lab Development, LLC
253-503-2560 office
253-312-9
New St. Augustine lab opening in January needs a fulltime HT or HTL with
Florida license. GI practice offers benefits, very flexible hours, and ideal
working conditions. Must have 3 to 5 years experience. Please contact me if you
are interested.
Konni Black
Pathology/ Lab Development, LLC
253-5
What kind of decal are you using? If it is HCL based, it may be too
harsh.
I wouldn't broadcast the B5 fact to the world!! :>)
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax
-Original Mess
Fellow Histonetters,
Is there any one using the Leica BOND IHC instrument who is having trouble
making their Kappa and Lambda work well on their bone marrow specimens? We
are presently using B5 on our bone marrows. Does anybody who uses B5 have
this problem? Thanks for your help.
Deanne Knu
Please Akemi, give me a break!
"Dear Valued Member"? Don't they even know WHO you are?
It is the same as receiving a junk mail addressed to you or to the "Current
Resident", they don't know.
You should not have answered that e-mail. Flag it spam and move on with your
e-mail!
René J.
--- On Thu,
Well Guys, looks like I have definitely been Scammed and Spammed! After I
thought about the possible rouse, I did however copy the original Alert that
was sent and sent it to the Yahoo Alert Center. The REAL Yahoo Alert Center
has the CRIMINAL'S e-mail addresses to do their investigation.
Ake
Here is helpful information from Yahoo. Akemi you will want to contact
Yahoo ASAP.
http://safely.yahoo.com/faq
Victor Tobias
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
vic...@pathology.washington.edu
206-5
You can get them from VWR (item #: 70690-024). 16 slides per page
Justin Peters, HTL (ASCP)
IHC Supervisor
Bostwick Laboratories(tm)
For Absolute Confidence(r)
4355 Innslake Drive
Glen Allen, Virginia 23060
Phone:(804) 967-9225 ext. 1831
Cell:(804) 822-6084
Email: jpet...@bostwickla
Liz,
Fisher Scientific carries this product 22088724
Walter Benton, HT(ASCP)QIHC
Histology Supervisor
University of Maryland Medical Center
Anatomic Pathology
22 S. Greene St
Room NBW65
Baltimore MD 21201
(Direct) 410-328-0930
(Lab) 410-328-5524
(Fax) 410-328-5508
This e-mail and any acc
This is the Yahoo ALERT that was sent it looks legitimate;From:
basant...@yahoo.com
Subject:Dear Valued Member,
Date: November 11, 2009 10:49:15 AM PST
To: undisclosed recipients: ;
Reply-To: messagecenter2...@live.comaccount Alert
Dear Valued Member,
Yahoo Mail has disc
The information they wanted was my user name, password and birthday
You should NEVER give out this info - if it is legit Yahoo would have
what they need to investigate. I think you are being scammed.
Andi
Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cell B
Phishing also happens over the phone as well. It is not unheard of that
people get phone calls claiming to be from a bank asking for personal
information (why would a bank need your account number... aren't they your
bank and don't they already have this information?). It is generally a very
bad id
Akemi:
And that was your second mistake. Why the server, in this case Yahoo, would
like your password and your birth day when that is not originally needed?
I NEVER EVER answer anything even if I am told that my life depends on it.
Never answer a request for information of any kind. If it a legiti
This happened to one my techs too with his hotmail account. It took him a lot
of time to prove to hotmail what had happened and to get his account reopened.
He was supposedly in London and needed money. Several of his friends called
him and that was how he was alerted to the scam. Be carefu
Akemi - Thanks for passing this on, even if it is legit. Good to be aware
of what's out there. I have heard of email viruses that will send out
emails from your account to contacts in your directory, (this would include
Histonet...)
Regards,
Merced
--On Thursday, November 12, 2009 11:15 AM -0
Both the Dako concentrate at 1: 300 with pH 6 target retrieval and the
Cell Marque rtu which is IVD not ASR by the way with ph 6.0 traget
retrieval are very clean also.
From: godsgal...@aol.com [mailto:godsgal...@aol.com]
Sent: Thursday, November 12, 2009 9:30
I hope this was legitimate! The information they wanted was my user name,
password and birthday.
yeah, sounds legit to me... lol...
Akemi Allison-Tacha wrote:
Hi All,
I received an e-mail and an early morning call from my friend Helen Hedor this morning stating that she received a e-mail
We are also trying Biocare's mouse monoclonal with red chromagen. Organisms
look like HP on steroids!
Beautiful...
Konni Black
- Original Message -
From:
To: ; ;
Sent: Thursday, November 12, 2009 7:30 AM
Subject: Re: [Histonet] Helicobacter pylori IHC stain
We use Biocare's Rab
Hi All,
I received an e-mail and an early morning call from my friend Helen Hedor this
morning stating that she received a e-mail scam from me stating; "FISHING FOR
MONEY". I did in NO WAY send this e-mail! Please do not in anyway answer or
send money to the bogus e-mail scam which was sent in
We use Biocare's Rabbit Polyclonal (1:150) with the red chromagenreally
cleaned up all the background..the docs love it!
Roxanne
-Original Message-
From: Taylor, Jean
To: 'ih...@googlegroups.com' ;
'histonet@lists.utsouthwestern.edu'
Sent: Tue, Nov 10, 2009 4:41 pm
Subject:
I am having a difficult time finding two antibodies I need. Two companies I
ordered them from have discontinued them.
VEGF and FGF-2
The tissue is human. Monoclonals preferred but at this point I'll use
polyclonals if necessary.
Does anyone know a vendor who has them?
Thanks.
__
We have a job opening here at Allergan Inc. in Irvine CA. You can find
the job posting on the Allergan web page or the NSH jobs site.
Thanks,
John
This e-mail, including any attachments, is meant only for the intended
recipient and may be a confidential communication or a communication
I also receive one of the scam emails.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara
Sent: Thursday, November 12, 2009 9:21 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Akem
Akemi Allison-Tacha - email me immediately, please. I think you are
being scammed and I don't want to email you directly.
Sally Breeden, HT(ASCP)
NM Dept. of Agriculture
Veterinary Diagnostic Services
PO Box 4700
Albuquerque, NM 87106
505-841-2576
Good Morning-
Need some advice for this stain. We are having trouble with severely weakened
stain. We run on the Leica Bond using Epitope Retrieval 2 for 30 minutes. We
recently changed from ER2/20 and saw improvement. Ran a trial on stock control
blocks (which are kept refrigerated) and on
Andrew,
I will be updating the Hard Tissue Committee members regarding this
very soon but this is very funny that the subject has come up yet
again. In fact, I have received a few phone calls over the past couple
of weeks on the subject and ironically two yesterday. Addittionally,
this wa
I'm looking for a new supplier for Sanderson's Rapid Bone Stain as Surgipath
have stopped selling it (at least in Europe).
Does anyone know of an alternative supplier or an equivalent stain I could
substitute in?
[Currently use the RBS on 15µm ground Technovit 7200VLC resin sections, with
acid
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