I will be out of the office starting 11/23/2009 and will not return until
11/30/2009.
.In my absence please ask for Mary Campbell . If this is urgent you can
contact me on my cell phone number 858-472-4266.
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Kaiser Regional Laboratory in Berkeley, CA has 4 full time Histotech openings,
if interested please call Kiranjit @ 510-559-5404.
--- On Mon, 11/16/09, Isaac O wrote:
From: Isaac O
Subject: [Histonet] NEW POSITION WANTED PLS
To: histonet@lists.utsouthwestern.edu
Date: Monday, November 16,
Help! I obtained an old Tissue-Tek II 4640 tissue processor, but I need the
baskets, and a manual, if possible!
Patty Lott, Laboratory Manager
UAB CMBD Core Laboratory 205-934-2007
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Sorry Guys I was thinking of IL-6 which I have done on bone and it
works. We have worked with TNF-alpha (from Sigma) and our protocol does
not requite retrieval I would think it would work on formic acid
decalcified bone also, but I have not tried it.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
I have a full time position open for a histotech at NAMSA, located just south
and east of Toledo in Northwood, Ohio. This is a full time days position, and
requires skills in embedding, sectioning, staining, etc. Must be willing to
work on animal tissues. We work on many interesting specimens
Richard it works. We purchase the antibody from Abcam.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949
fax (303) 682-9060
www.premierlab.com
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorad
I have a colleague who is interested in doing IHC for TNF alpha on
formalin-fixed, decalcified bone specimens. Is this possible? If so, I would
appreciate hearing about your antibody and technique. Thanks!
Richard
Richard W. Cartun, Ph.D.
Director, Histology & Immunopathology
Director, Biosp
uthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Altho' retired for 2 years, we had a protocol that retained all parts of the
eye quite well. Fixation was in 3% glutaraldehyde (diluted in H2O), 4 deg.C
for overnight. Don't extend the fixation as this will cause the tissue to be
too hard. Wash for about 1hr in running tap water and process a
Hi all,
I have an opening for a Histotech III in the histology lab which (it was just
posted). For this position, an HTL is required, no exceptions. We are looking
for someone with 5+ years of experience in routine histology, manual specials,
automated special stain experience is always a hel
RE: Cryojane Tape Transfer System
I have found that you need to have your tape at the same temp as your
tissue to have your tissue transfer completely from the tape. I set
everything up in the morning putting the tape in the cryostat and get
everything equilibrated first for about 2 hours or so.
Hello, Richard,
I have done some sectioning of undecalcified bone with the Cryojane
(hallelujah! it works! to paraphrase Ms. Callis) and one thing is
that the sections won't cross-link if the tapes or the slides are
being stored in the presence of UV light (then they are
pre-crosslinked!), ke
You wrote:
Unless I'm missing something, I don't understand why people use this tape?
It seems like a marketing gimmick to me...ol' fashion' melting of sections
onto slides works perfectly for us...
?
Regards,
Merced
Merced,
Yes, you are missing something. If you have ever tried
Hi Richard,
I am assuming that you are using the flash in the cryojane system to adhere the
specimen to the slide. Make sure your tape is not too old and don't store it in
the cryostat. It sounds like you are able to get the section but unable to get
it to adhere. Make sure you pass your rol
We have an opening for a tech, days no weekends at St Pete.General Hospital in
St Pete,FL. Call our HR dept at 727 384-1414 X 4814.
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My question concerns mouse eyes.
Can anyone send me their fixation suggestions? We are receiving mouse eyes
that end up with a "wavy" look instead of a nicely defined "C" and have no
explanation for this occurrence.
Can anyone send me their mouse eye processing schedule? We have a protocol
t
Good Monday morning,
One of our pathologists is getting pressure from the endocrinologists to
bring on Parafibromin (gene: hrpt2). Are there any reference labs out
there offering this?
Thanks,
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland
That seems like a lot of effort. Do your pathologists do the grossing? or PA's?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Fye Beth
Sent: Monday, November 23, 2009 9:38 AM
To: histonet@lists.utsouthwest
There have been some great suggestions for reducing or catching slide labeling
errors. I'm interested in the same for cassette labeling. Currently, we have
the patient's last name on one side of the cassette, and the site listed on the
other. This makes it easier to identify blocks if they ar
Unless I'm missing something, I don't understand why people use this tape?
It seems like a marketing gimmick to me...ol' fashion' melting of sections
onto slides works perfectly for us...
?
Regards,
Merced
--On Monday, November 23, 2009 9:01 AM -0500 Richard Pattison
wrote:
Hi Everybody,
Hi Everybody,
I was hoping to get some advice - I'm cryosectioning plant tissues and
transferring sections to slides using the Cryojane system. However, i'm
having problems in transferring the sections without them falling apart
during the tape transfer. I'm fixing my tissue for 24 hours in
et
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