Folks:
Subject line says it all - - I'm especially interested in finding out
how it works and how the glass is scored
As always my thanks!
Mike Folsom
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e are
several vendors, Dorn and Hart, DDK, and probably microtome manufacturers
too.
Gayle Callis
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Any comments? thanks in advance
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You answered most of your own questions.
The bone should be fixed before decalcifying it.
After that it could be cut in the cryostat, but why to do that?
It will be quite difficult. The best solution is to fix → decalcify → process
routine by embedding in paraffin → cut "conventional" sections.
Th
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Question regarding fresh tissue, in this case bone.
Scenario:
Mohs surgeon removes layers of skin on a patient's scalp,
eventually discovering the basal cell carcinoma extends to
the periosteum and so a bit of bone is taken and tested to
ensure the margins are free of cancer. The only problem is
Thomas Jasper certainly points out an issue of politeness: >>Being
chastised on this list and calling your work "bad science" is totally
out of line and certainly does not help you out.<<
I agree up to a point - but frankly, if John Kiernan called my work
"bad science" I would seriously reconsider
Just about all biology research institutions have an animal unit with
qualified technicians to look after the animals, rather than someone
qualified in other areas; however the technicians are subject to
human failings such as going home at night. I've never heard of a one-
person research t
Hi Histonetters
Who out there has a Sakura iDent?
I have just taken delivery of a brand new machine and I am having serious
issues with the quality of the print on the cassette face - the ink rubs off
with xylene!!!
Yes, I leave the print to dry before I handle the cassettes
No, I dont use Tissue
Nate,
I think you have confused the persons. I'm the one, who recommended longer
fixation and Kris had made the original question.
Regards
Gudrun
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